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中国农学通报 ›› 2019, Vol. 35 ›› Issue (13): 64-69.doi: 10.11924/j.issn.1000-6850.casb18110127

所属专题: 生物技术 园艺

• 林学 园艺 园林 • 上一篇    下一篇

甜瓜果皮颜色遗传分析及基因定位

欧点点, 赵光伟, 贺玉花, 王平勇, 徐志红, 孔维虎, 张健, 徐永阳   

  1. 中国农业科学院郑州果树研究所甜瓜遗传育种课题组
  • 收稿日期:2018-11-29 修回日期:2019-04-07 接受日期:2019-03-15 出版日期:2019-05-05 发布日期:2019-05-05
  • 通讯作者: 徐永阳
  • 基金资助:
    国家西甜瓜产业技术体系“种质资源收集与评价岗位”(CARS-25);中国农业科学院科技创新工程“西甜瓜资源与育种”(CAAS-ASTIP- 2016ZFRI-06);中央级科研院所基本科研业务费专项“甜瓜皮色相关性状的基因定位研究”(1610192018107)。

Genetic Analysis and Gene Mapping for Melon Rind Color

  • Received:2018-11-29 Revised:2019-04-07 Accepted:2019-03-15 Online:2019-05-05 Published:2019-05-05

摘要: 为探究甜瓜皮色性状的遗传规律,定位目标性状关联的遗传位点,选用黄皮材料B432 和绿皮材料B168、B421 为亲本,构建6 世代遗传群体(P1、P2、F1、F2、BC1P1、BC1P2),用于分析甜瓜皮色性状的遗传规律;同时,利用BSA-seq 和全基因组重测序技术,定位控制甜瓜皮色的基因。结果表明,甜瓜皮色由2对基因控制,其中绿色对白色具有显性上位性效应,白色对黄色为显性。同时,将皮色相关基因分别定位于4 号染色体和10 号染色体的0.02~5.7 Mb和0.08~9.5 Mb区间。试验初步定位了控制甜瓜皮色的基因,为后续进行基因精细定位提供依据,为开展甜瓜皮色的分子标记辅助选择奠定基础。

关键词: 海南岛、强冷空气、大气环流、强降温、降水, 海南岛、强冷空气、大气环流、强降温、降水

Abstract: To explore the inheritance and genetic loci of melon rind color, the yellow-rind line B432 and green-rind lines B168 and B421 were used as parent to construct six generations of genetic population (P1, P2, F1, F2, BC1P1, BC1P2) to analyze the inheritance of melon rind color. In addition, the BSA-seq and whole genome resequencing technology were used to locate the genetic loci responsible for melon rind color. The results show that the melon rind color is controlled by two genes, of which green has a dominant epistatic effect on white and white is dominant to yellow. The rind color related genes are mapped to the 0.02-5.7 Mb and 0.08- 9.5 Mb intervals of chromosome 4 and chromosome 10, respectively. This experiment preliminarily elucidates the genetic mechanism and the location of the genes controlling the rind color of melon, and could provide a basis for the subsequent fine-grained gene mapping and molecular marker-assisted selection of melon rind color.