中国农学通报 ›› 2020, Vol. 36 ›› Issue (11): 56-62.doi: 10.11924/j.issn.1000-6850.casb18120069
收稿日期:
2018-12-18
修回日期:
2019-06-17
出版日期:
2020-04-15
发布日期:
2020-04-28
通讯作者:
李杰
作者简介:
王仁睿,女,1981年出生,四川都江堰人,讲师,博士,研究方向:园艺植物超低温保存及脱毒研究。通信地址:621000 四川省绵阳市涪城区青龙大道59号 西南科技大学生命科学与工程学院,Tel:0816-6089534,E-mail: renruiwang@nwsuaf.edu.cn, 695806986@qq.com
基金资助:
Received:
2018-12-18
Revised:
2019-06-17
Online:
2020-04-15
Published:
2020-04-28
Contact:
Li Jie
摘要:
为了弄清目前建兰花叶病毒(CymMV)和齿兰环斑病毒(ORSV)病毒检测和脱毒的研究概况,本研究详细阐述了2种病毒的检测方法、采用的脱毒技术和脱毒效率,分析比较了不同技术存在的弊端,指出现有的脱毒方法已不能满足兰科植物脱毒研究的需要。在此基础上,进一步提出应采用安全、可靠的新型脱毒技术来解决目前兰科植物产业瓶颈问题。超低温脱毒技术是近年来发展迅速的脱毒技术,具有独特优势,目前已在多种植物上成功应用,该方法的应用有望解决2种病毒脱毒方面存在的问题。
中图分类号:
王仁睿, 李杰. 建兰花叶病毒和齿兰环斑病毒的检测技术及脱毒方法研究进展[J]. 中国农学通报, 2020, 36(11): 56-62.
Wang Renrui, Li Jie. Cymbidium mosaic virus and Odontoglossum ringspot virus: Research Progress on Detection Techniques and Virus Elimination[J]. Chinese Agricultural Science Bulletin, 2020, 36(11): 56-62.
植物种类 | 病原体 | 脱毒率/% | 参考文献 | ||
---|---|---|---|---|---|
茎尖培养 | 超低温疗法 | ||||
李Prunus | 李痘病毒 | 10 | 50 | [51] | |
香蕉Musa spp. | 黄瓜花叶病毒 | 0 | 30 | [52] | |
香蕉条斑病毒 | 76 | 90 | |||
香蕉束顶病毒 | 26.7 | 60.6 | [53] | ||
葡萄Vitis vinifera | 葡萄A病毒 | 12 | 96 | [54] | |
42 | [55] | ||||
马铃薯Solanum tuberosum | 马铃薯卷叶病毒 | 56 | 85 | [56] | |
马铃薯Y病毒 | 62 | 93 | |||
马铃薯卷叶病毒 | 14 | 37 | [57] | ||
马铃薯Y病毒 | 11 | 42 | |||
马铃薯X病毒 | 36 | 59.1 | [58] | ||
树莓Rubus idaeus | 树莓丛状矮化病毒 | 0 | 35 | [59] | |
甘薯Ipomoea batatas | 甘薯褪绿矮化病毒 | 100 | 100 | [60] | |
甘薯羽状斑驳病毒 | 10 | 100 | |||
2种病毒复合感染 | 7 | 100 | |||
甘薯小叶植原体 | 10 | 100 | [61] | ||
柑 Citus spp. | 柑橘黄龙病 | 25 | 98 | [62] | |
柑橘裂皮病类病毒 | 88.2-95.2 | [63] | |||
山药Dioscorea opposita | 山药花叶病毒 | 40 | 90 | [64] | |
朝鲜蓟Cynara cardunculus | 朝鲜蓟潜隐病毒 | 100 | [65-66] | ||
苹果Malus pumila | 苹果茎痘病毒 | 0~10 | 80-85 | [67] | |
苹果褪绿叶斑病毒 | 0 | 100 | [68] | ||
苹果茎沟病毒 | 0 | 10 | [69] | ||
草莓Fragaria ananassa | 草莓斑驳病毒 | 85 | 100 | [70] | |
草莓轻型黄边病毒 | |||||
草莓镶脉病毒 | |||||
草莓皱缩病毒 | |||||
草莓轻型黄边病毒 | 0 | 95 | [71] | ||
大蒜Allium sativum | 洋葱黄矮病毒 | 26.1 | 87.5 | [72] | |
韭菜黄带病毒 | 34.7 | 93.8 | |||
大蒜潜伏病毒 | 13.0 | 62.5 | |||
菊花Chrysanthemum morifolium | 菊花B病毒 | 0 | 21.9-30.8 | [73] | |
枣Ziziphus jujuba | 枣疯病 | 0-7 | 100 | [74] | |
红芽芋 | 芋花叶病毒 | 75 | 100 | [75] |
植物种类 | 病原体 | 脱毒率/% | 参考文献 | ||
---|---|---|---|---|---|
茎尖培养 | 超低温疗法 | ||||
李Prunus | 李痘病毒 | 10 | 50 | [51] | |
香蕉Musa spp. | 黄瓜花叶病毒 | 0 | 30 | [52] | |
香蕉条斑病毒 | 76 | 90 | |||
香蕉束顶病毒 | 26.7 | 60.6 | [53] | ||
葡萄Vitis vinifera | 葡萄A病毒 | 12 | 96 | [54] | |
42 | [55] | ||||
马铃薯Solanum tuberosum | 马铃薯卷叶病毒 | 56 | 85 | [56] | |
马铃薯Y病毒 | 62 | 93 | |||
马铃薯卷叶病毒 | 14 | 37 | [57] | ||
马铃薯Y病毒 | 11 | 42 | |||
马铃薯X病毒 | 36 | 59.1 | [58] | ||
树莓Rubus idaeus | 树莓丛状矮化病毒 | 0 | 35 | [59] | |
甘薯Ipomoea batatas | 甘薯褪绿矮化病毒 | 100 | 100 | [60] | |
甘薯羽状斑驳病毒 | 10 | 100 | |||
2种病毒复合感染 | 7 | 100 | |||
甘薯小叶植原体 | 10 | 100 | [61] | ||
柑 Citus spp. | 柑橘黄龙病 | 25 | 98 | [62] | |
柑橘裂皮病类病毒 | 88.2-95.2 | [63] | |||
山药Dioscorea opposita | 山药花叶病毒 | 40 | 90 | [64] | |
朝鲜蓟Cynara cardunculus | 朝鲜蓟潜隐病毒 | 100 | [65-66] | ||
苹果Malus pumila | 苹果茎痘病毒 | 0~10 | 80-85 | [67] | |
苹果褪绿叶斑病毒 | 0 | 100 | [68] | ||
苹果茎沟病毒 | 0 | 10 | [69] | ||
草莓Fragaria ananassa | 草莓斑驳病毒 | 85 | 100 | [70] | |
草莓轻型黄边病毒 | |||||
草莓镶脉病毒 | |||||
草莓皱缩病毒 | |||||
草莓轻型黄边病毒 | 0 | 95 | [71] | ||
大蒜Allium sativum | 洋葱黄矮病毒 | 26.1 | 87.5 | [72] | |
韭菜黄带病毒 | 34.7 | 93.8 | |||
大蒜潜伏病毒 | 13.0 | 62.5 | |||
菊花Chrysanthemum morifolium | 菊花B病毒 | 0 | 21.9-30.8 | [73] | |
枣Ziziphus jujuba | 枣疯病 | 0-7 | 100 | [74] | |
红芽芋 | 芋花叶病毒 | 75 | 100 | [75] |
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