2种花色的薰衣草花色苷成分分析

doi:10.11924/j.issn.1000-6850.casb2022-0630

摘要/Abstract

摘要：

为明确2种不同花色薰衣草间花色苷成分及含量差异性，探索薰衣草花色与花色苷的相关性。以‘新薰二号’（蓝花）和‘YXA-05’（白花）2种薰衣草花器官为研究材料，利用超高效液相色谱－四极杆飞行时间质谱(UPLC-Q-TOF/MS)联用技术测定2种薰衣草花器官中花色苷的成分及含量。结果表明，‘新薰二号’和‘YXA-05’2种薰衣草花冠中总花色苷含量分别为42.24 μg/g和35.96 μg/g，均含有6类花色苷，分别为矢车菊素、芍药花素、飞燕草素、矮牵牛素、锦葵素和天竺葵素。其中‘新薰二号’含有39种花色苷，‘YXA-05’含有37种花色素苷，‘新薰二号’含有6种‘YXA-05’中不存在的花色苷，而‘YXA-05’含有4种‘新薰二号’中不存在的花色苷。可见，2种薰衣草中花色苷的种类丰富，且成分及含量存在差异，薰衣草花冠的颜色与其花色素苷的成分和含量有关。

关键词: 超高效液相色谱－四极杆－飞行时间质谱, ‘新薰二号’, 花色苷, 飞燕草素, 矮牵牛素

Abstract:

The aim is to clarify the composition and content differences of anthocyanin between two kinds of lavender varying in flower color, and to explore the relevance between lavender flower color and anthocyanin. The flower organs of ‘Xinxun No. 2’ (blue flower) and ‘YXA-05’ (white flower) were used as research materials. The composition and content of anthocyanins in flower organs of the two lavender varieties were determined by using the combination technology of ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF/MS). The result showed that the total anthocyanin content in ‘Xinxun No. 2’ and ‘YXA-05’ was 42.24 μg/g and 35.96 μg/g respectively, and both two varieties contained 6 types of anthocyanin, including cyanidin, peonidin, delphinidin, petunidin, malyidin and pelargonidin. There were 39 types of anthocyanin in ‘Xinxun No. 2’, and 37 types of anthocyanin in ‘YXA-05’. ‘Xinxun No. 2’ contained 6 types of anthocyanin that were not found in ‘YXA-05’, while ‘YXA-05’ contained 4 types of anthocyanin that were not found in ‘Xinxun No. 2’. It was indicated that in these two lavender varieties, anthocyanin was rich in types and differed in composition and content. The flower color of corolla was related to the composition and content of its anthocyanin.

Key words: ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry, ‘Lavender Xinxun No. 2’, anthocyanin, delphinidin, petunidin

赖刚刚, 李敏, 蒋新明, 徐盼盼, 李子玉, 郭丹丽. 2种花色的薰衣草花色苷成分分析[J]. 中国农学通报, 2023, 39(15): 39-45.

LAI Ganggang, LI Min, JIANG Xinming, XU Panpan, LI Ziyu, GUO Danli. Anthocyanin in Lavender of Two Flower Colors: Analysis of Compositions[J]. Chinese Agricultural Science Bulletin, 2023, 39(15): 39-45.

图/表 5

参考文献 26

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编号		苷元类型	化合物名称		含量/(μg/g)
编号		苷元类型	化合物名称		蓝花	白花
1		Cyanidin [矢车菊素]	Cyanidin-3,5-O-diglucoside[矢车菊素-3,5-O-二葡萄糖苷]		0.4463±0.0863	0.2100±0.0407
2			Cyanidin-3-O-glucoside[矢车菊素-3-O-葡萄糖苷]		0.0671±0.0013	0.0411±0.0021
3			Cyanidin-3-O-5-O-(6-O-coumaroyl)-diglucoside [矢车菊素-3-O-5-O-(6-O-对香豆酰)-二葡萄糖苷]		14.7591±3.364 9	7.6792±1.1506
4			Cyanidin-3,5,3'-O-triglucoside[矢车菊素-3,5,3'-O-三葡萄糖]		--	--
5			Cyanidin-3-O-arabinoside[矢车菊素-3-O-阿拉伯糖苷]		--	0.0137±0.0407
6			Cyanidin-3-O-sophoroside[矢车菊素-3-O-槐糖苷]		0.0143±0.0098	0.0143±0.0002
7		Delphinidin [飞燕草素]	Delphinidin-3-O-(6-O-malonyl)-glucoside-3'-glucoside [飞燕草素-3-O-(6-O-丙二酰基)-葡萄糖苷-3'-葡萄糖苷]		0.0522±0.0045	0.0177±0.0031
8			Delphinidin-3-O-glucoside[飞燕草素-3-O-葡萄糖苷]		0.0300±0.0029	0.0369±0.0041
9			Delphinidin-3,5-O-diglucoside[飞燕草素-3,5-O-二葡萄糖苷]		0.0215±0.0100	0.0152±0.0009
10			Delphinidin-3-O-(6-O-malonyl-beta-D-glucoside) [飞燕草素-3-O-(6-O-丙二酰-β-D-葡萄糖苷)]		0.2333±0.0255	0.0722±0.0067
11			Delphinidin-3-O-5-O-(6-O-coumaroyl)-diglucoside [飞燕草素-3-O-5-O-(6-O-对香豆酰)-二葡萄糖苷]		0.2394±0.0976	0.2460±0.0271
12			Delphinidin-3-O-galactoside[飞燕草素-3-O-半乳糖苷]		0.0519±0.0014	0.0254±0.0023
13			Delphinidin-3-O-rutinoside[飞燕草素-3-O-芸香糖苷]		0.0032±0.0006	0.0002±0.0002
14			Delphinidin-3-O-sophoroside[飞燕草素-3-O-槐糖苷]		0.1639±0.0064	0.0832±0.0012
15			Delphinidin[飞燕草素]		0.0029±0.0018	--
16		Malvidin [锦葵色素]	Malvidin-3,5-O-diglucoside[锦葵色素-3,5-O-二葡萄糖苷]		0.0561±0.0032	0.0244±0.0021
17			Malvidin-3-O-glucoside[锦葵色素-3-O-葡萄糖苷]		0.0334±0.0006	0.0174±0.0011
18			Malvidin-3-O-(6'-acetylglucoside)-5-glucoside [锦葵色素-3-O-(6'-乙酰基葡萄糖苷)-5-葡萄糖苷]		0.0067±0.0009	0.0041±0.0006
19			Malvidin-3-O-(6-O-p-coumaroyl)-glucoside[锦葵色素-3-(6-O-p-对香豆酰)-葡萄糖苷]		0.1877±0.0102	0.1131±0.0076
20			Malvidin-3-O-5-O-(6-O-coumaroyl)-diglucoside [锦葵色素-3-O-5-O-(6-O-对香豆酰)-二葡萄糖苷]		1.0690±0.0663	0.7004±0.0222
21			Malvidin-3-O-sophoroside[锦葵色素-3-O-槐糖苷]		0.0049±0.0012	0.0016±0.0006
22			Malvidin-3-O-(6-O-malonyl-beta-D-glucoside)[锦葵色素-3-O-6-O-丙二酰-β-D-葡萄糖苷]		0.0125±0.0017	--
23	Pelargonidin [天竺葵素]			Pelargonidin-3,5-O-diglucoside[天竺葵素-3,5-O-二葡萄糖苷]	0.0302±0.0080	0.0290±0.0162
24				Pelargonidin-3-O-(6-O-p-coumaroyl)-glucoside[天竺葵素-3-(6-O-p-对香豆酰)-葡萄糖苷]	1.1413±0.7978	1.6203±0.3566
25				Pelargonidin-3-O-glucoside[天竺葵素-3-O-葡萄糖苷]	2.5678±0.0492	3.1926±0.0845
26				Pelargonidin-3-O-sophoroside[天竺葵素-3-O-槐糖苷]	0.0119±0.0013	0.0191±0.0022
27				Pelargonidin-3-O-sophoroside-5-O-(malonyl)-glucoside [天竺葵素-3-O-槐糖苷-5-O-(丙二酰基)-葡萄糖苷]	0.0027±0.0006	0.0034±0.0002
28				Pelargonidin-3-O-(6-O-malonyl-beta-D-glucoside) [天竺葵素-3-O-(6-O-丙二酰-β-D-葡萄糖苷)]	17.9282±0.2704	21.2398±0.1752
29				Pelargonidin-3-O-galactoside[天竺葵素-3-O-半乳糖苷]	0.1132±0.0130	0.1027±0.0199
30				Pelargonidin-3-O-rutinoside-5-O-glucoside[天竺葵素-3-O-芸香糖苷-5-O-葡萄糖苷]	0.0085±0.0007	0.013 0±0.0011
31	Peonidin [芍药花素]			Peonidin-3,5-O-diglucoside[芍药花素-3,5-O-二葡萄糖苷]	0.0360±0.0008	0.0440±0.0009
32				Peonidin-3-O-(6-O-p-coumaroyl)-glucoside[芍药花素-3-(6-O-p-对香豆酰)-葡萄糖苷]	0.0377±0.0012	0.0559±0.0089
33				Peonidin-3-O-5-O-(6-O-coumaroyl)-diglucoside [芍药花素-3-O-5-O-(6-O-对香豆酰)-二葡萄糖]	0.2123±0.0189	0.2912±0.0089
34				Peonidin-3-O-glucoside[芍药花素-3-O-葡萄糖苷]	0.0058±0.0007	0.0152±0.0005
35				Peonidin-3-O-galactoside[芍药花素-3-O-半乳糖苷]	0.0415±0.0050	--
36				Peonidin-3-O-(6-O-malonyl-beta-D-glucoside) [芍药花素-3-O-(6-O-丙二酰-β-D-葡萄糖苷)]	--	0.0027±0.0002
37	Petunidin [矮牵牛素]			Petunidin-3-O-glucoside[矮牵牛素-3-O-葡萄糖苷]	0.0101±0.0006	0.0168±0.0006
38				Petunidin-3-O-(6-O-p-coumaroyl)-glucoside[矮牵牛素-3-(6-O-p-对香豆酰)-葡萄糖苷]	0.4112±0.1098	0.1144±0.0347
39				Petunidin-3-O-5-O-(6-O-coumaroyl)-diglucoside [矮牵牛素-3-O-5-O-(6-O-对香豆酰)-二葡萄糖苷]	2.1766±0.5116	0.6456±0.1385
40				Petunidin-3-O-rutinoside[矮牵牛素-3-O-芸香糖苷]	0.0131±0.0012	0.0042±0.0009
41				Petunidin-3,5-O-diglucoside[矮牵牛素-3,5-O-二葡萄糖苷]	0.0218±0.0028	--
42				Petunidin-3-O-galactoside[矮牵牛素-3-O-半乳糖苷]	0.0301±0.0068	--
43				Petunidin-3-O-(6-O-malonyl-beta-D-glucoside) [矮牵牛素-3-O-(6-O-丙二酰-β-D-葡萄糖苷)]	--	0.0200±0.0052

编号		苷元类型	化合物名称		含量/(μg/g)
编号		苷元类型	化合物名称		蓝花	白花
1		Cyanidin [矢车菊素]	Cyanidin-3,5-O-diglucoside[矢车菊素-3,5-O-二葡萄糖苷]		0.4463±0.0863	0.2100±0.0407
2			Cyanidin-3-O-glucoside[矢车菊素-3-O-葡萄糖苷]		0.0671±0.0013	0.0411±0.0021
3			Cyanidin-3-O-5-O-(6-O-coumaroyl)-diglucoside [矢车菊素-3-O-5-O-(6-O-对香豆酰)-二葡萄糖苷]		14.7591±3.364 9	7.6792±1.1506
4			Cyanidin-3,5,3'-O-triglucoside[矢车菊素-3,5,3'-O-三葡萄糖]		--	--
5			Cyanidin-3-O-arabinoside[矢车菊素-3-O-阿拉伯糖苷]		--	0.0137±0.0407
6			Cyanidin-3-O-sophoroside[矢车菊素-3-O-槐糖苷]		0.0143±0.0098	0.0143±0.0002
7		Delphinidin [飞燕草素]	Delphinidin-3-O-(6-O-malonyl)-glucoside-3'-glucoside [飞燕草素-3-O-(6-O-丙二酰基)-葡萄糖苷-3'-葡萄糖苷]		0.0522±0.0045	0.0177±0.0031
8			Delphinidin-3-O-glucoside[飞燕草素-3-O-葡萄糖苷]		0.0300±0.0029	0.0369±0.0041
9			Delphinidin-3,5-O-diglucoside[飞燕草素-3,5-O-二葡萄糖苷]		0.0215±0.0100	0.0152±0.0009
10			Delphinidin-3-O-(6-O-malonyl-beta-D-glucoside) [飞燕草素-3-O-(6-O-丙二酰-β-D-葡萄糖苷)]		0.2333±0.0255	0.0722±0.0067
11			Delphinidin-3-O-5-O-(6-O-coumaroyl)-diglucoside [飞燕草素-3-O-5-O-(6-O-对香豆酰)-二葡萄糖苷]		0.2394±0.0976	0.2460±0.0271
12			Delphinidin-3-O-galactoside[飞燕草素-3-O-半乳糖苷]		0.0519±0.0014	0.0254±0.0023
13			Delphinidin-3-O-rutinoside[飞燕草素-3-O-芸香糖苷]		0.0032±0.0006	0.0002±0.0002
14			Delphinidin-3-O-sophoroside[飞燕草素-3-O-槐糖苷]		0.1639±0.0064	0.0832±0.0012
15			Delphinidin[飞燕草素]		0.0029±0.0018	--
16		Malvidin [锦葵色素]	Malvidin-3,5-O-diglucoside[锦葵色素-3,5-O-二葡萄糖苷]		0.0561±0.0032	0.0244±0.0021
17			Malvidin-3-O-glucoside[锦葵色素-3-O-葡萄糖苷]		0.0334±0.0006	0.0174±0.0011
18			Malvidin-3-O-(6'-acetylglucoside)-5-glucoside [锦葵色素-3-O-(6'-乙酰基葡萄糖苷)-5-葡萄糖苷]		0.0067±0.0009	0.0041±0.0006
19			Malvidin-3-O-(6-O-p-coumaroyl)-glucoside[锦葵色素-3-(6-O-p-对香豆酰)-葡萄糖苷]		0.1877±0.0102	0.1131±0.0076
20			Malvidin-3-O-5-O-(6-O-coumaroyl)-diglucoside [锦葵色素-3-O-5-O-(6-O-对香豆酰)-二葡萄糖苷]		1.0690±0.0663	0.7004±0.0222
21			Malvidin-3-O-sophoroside[锦葵色素-3-O-槐糖苷]		0.0049±0.0012	0.0016±0.0006
22			Malvidin-3-O-(6-O-malonyl-beta-D-glucoside)[锦葵色素-3-O-6-O-丙二酰-β-D-葡萄糖苷]		0.0125±0.0017	--
23	Pelargonidin [天竺葵素]			Pelargonidin-3,5-O-diglucoside[天竺葵素-3,5-O-二葡萄糖苷]	0.0302±0.0080	0.0290±0.0162
24				Pelargonidin-3-O-(6-O-p-coumaroyl)-glucoside[天竺葵素-3-(6-O-p-对香豆酰)-葡萄糖苷]	1.1413±0.7978	1.6203±0.3566
25				Pelargonidin-3-O-glucoside[天竺葵素-3-O-葡萄糖苷]	2.5678±0.0492	3.1926±0.0845
26				Pelargonidin-3-O-sophoroside[天竺葵素-3-O-槐糖苷]	0.0119±0.0013	0.0191±0.0022
27				Pelargonidin-3-O-sophoroside-5-O-(malonyl)-glucoside [天竺葵素-3-O-槐糖苷-5-O-(丙二酰基)-葡萄糖苷]	0.0027±0.0006	0.0034±0.0002
28				Pelargonidin-3-O-(6-O-malonyl-beta-D-glucoside) [天竺葵素-3-O-(6-O-丙二酰-β-D-葡萄糖苷)]	17.9282±0.2704	21.2398±0.1752
29				Pelargonidin-3-O-galactoside[天竺葵素-3-O-半乳糖苷]	0.1132±0.0130	0.1027±0.0199
30				Pelargonidin-3-O-rutinoside-5-O-glucoside[天竺葵素-3-O-芸香糖苷-5-O-葡萄糖苷]	0.0085±0.0007	0.013 0±0.0011
31	Peonidin [芍药花素]			Peonidin-3,5-O-diglucoside[芍药花素-3,5-O-二葡萄糖苷]	0.0360±0.0008	0.0440±0.0009
32				Peonidin-3-O-(6-O-p-coumaroyl)-glucoside[芍药花素-3-(6-O-p-对香豆酰)-葡萄糖苷]	0.0377±0.0012	0.0559±0.0089
33				Peonidin-3-O-5-O-(6-O-coumaroyl)-diglucoside [芍药花素-3-O-5-O-(6-O-对香豆酰)-二葡萄糖]	0.2123±0.0189	0.2912±0.0089
34				Peonidin-3-O-glucoside[芍药花素-3-O-葡萄糖苷]	0.0058±0.0007	0.0152±0.0005
35				Peonidin-3-O-galactoside[芍药花素-3-O-半乳糖苷]	0.0415±0.0050	--
36				Peonidin-3-O-(6-O-malonyl-beta-D-glucoside) [芍药花素-3-O-(6-O-丙二酰-β-D-葡萄糖苷)]	--	0.0027±0.0002
37	Petunidin [矮牵牛素]			Petunidin-3-O-glucoside[矮牵牛素-3-O-葡萄糖苷]	0.0101±0.0006	0.0168±0.0006
38				Petunidin-3-O-(6-O-p-coumaroyl)-glucoside[矮牵牛素-3-(6-O-p-对香豆酰)-葡萄糖苷]	0.4112±0.1098	0.1144±0.0347
39				Petunidin-3-O-5-O-(6-O-coumaroyl)-diglucoside [矮牵牛素-3-O-5-O-(6-O-对香豆酰)-二葡萄糖苷]	2.1766±0.5116	0.6456±0.1385
40				Petunidin-3-O-rutinoside[矮牵牛素-3-O-芸香糖苷]	0.0131±0.0012	0.0042±0.0009
41				Petunidin-3,5-O-diglucoside[矮牵牛素-3,5-O-二葡萄糖苷]	0.0218±0.0028	--
42				Petunidin-3-O-galactoside[矮牵牛素-3-O-半乳糖苷]	0.0301±0.0068	--
43				Petunidin-3-O-(6-O-malonyl-beta-D-glucoside) [矮牵牛素-3-O-(6-O-丙二酰-β-D-葡萄糖苷)]	--	0.0200±0.0052