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中国农学通报

中国农学通报 ›› 2003, Vol. 19 ›› Issue (5): 12-12.

所属专题： 生物技术； 畜牧兽医

• 目次 • 上一篇    下一篇

从小鼠单个植入前胚胎中扩增线粒体DNA研究

李军锋,钱敏,鲁成,张家骅   

  • 出版日期:2003-10-25 发布日期:2003-10-25

Amplification of Mitochondria DNA from Mouse Single Preimplantation Embryo

Li Jun-feng Qian Min Lu Cheng Zhang Jia-hua   

  • Online:2003-10-25 Published:2003-10-25

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摘要： 用KOH/DTT对小鼠单个卵子、2-cell胚、8-cell胚、桑葚胚和囊胚进行处理，分别作为模板DNA，并用PCR技术对其mtDNA的高变异区D-loop区的5'端342bp片段进行扩增。结果，小鼠单个卵子、2-cell胚、8-cell胚、桑葚胚或囊胚经KOH/DTT处理后，紫外分光光度法测得DNA含量为10~10.75mg/mL，OD260/OD280值为0.835~0.944；以这种DNA样品为模板，经一次PCR扩增即能获得清晰的目的DNA条带。

关键词: 接骨木、茎段、组织培养, 接骨木、茎段、组织培养

Abstract: We have cultivated these cells from mice by KOH/DTT, such as single ovum, 2-cell embryo,8-cell embryo, morula and blastocyst. Then 5′ D-loop of mtDNA of these cells, which have 342bp, have been amplification by PCR technology. As a result, after these cells have cultivated by KOH/DTT, density of DNA, which has been tested by the mass UV-spectrophotometer, is 10~10.75mg/mL and OD260/OD280 is 0.835~0.944. Act these DNA as template DNA respectively, clear target DNA fragment could be attained by only one PCR amplification.