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中国农学通报

中国农学通报 ›› 2005, Vol. 21 ›› Issue (1): 101-101.

所属专题： 生物技术

• 目次 • 上一篇    下一篇

山新杨组织培养快繁技术研究

刘文萍,韩玉琴,南相日,朱 财   

  • 出版日期:2005-01-05 发布日期:2005-01-05

Study on Rapid Multiplication Techniques by Tissue Culture in Shanxinyang(P.davidiana X P.bollena )

Liu Wenping, Han Yuqin, Nan Xiangri, Zhu Cai   

  • Online:2005-01-05 Published:2005-01-05

摘要： 山新杨冬季枝条经FC处理，选择萌发新枝的茎段和叶片为接种外植体；诱导茎段产生腋芽的培养基为1/2MS+6-BA0.2~0.5mg/L+NAA0~0.5mg/L，诱导叶片产生不定芽的培养基为1/2MS或MS+6-BA0.5mg/L+NAA0.5mg/L；丛生芽快繁培养基MS+6-BA0.3mg/L+NAA0.3mg/L，每20天继代一次，繁殖倍数20左右；生根培养基为1/2MS或MS，附加0.1~0.5mg/L的多效唑，对生根有促进作用。

Abstract: Stem segments and leaves of new shooting, which are from winter shoot treated by FC , was used as inoculating explant. The medium used for producing axillary bud from stem segment is 1/2MS+6-BA0.2~0.5mg/L + NAA 0~0.5mg/L; the medium used for generating adventitious bud is 1/2MS or MS + 6-BA 0.5mg/L + NAA0.5mg/L; medium used for producing rosette buds is MS+6-BA0.3mg/L + NAA0.3mg/L. Regeneration was conducted every 20 days and 20 times of multiplication could be achieved. Rooting medium is 1/2MS or MS. Adding benzimidazole 0.1~0.5mg/L in the medium could promote rooting.