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中国农学通报

中国农学通报 ›› 2005, Vol. 21 ›› Issue (5): 113-113.

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兰州百合微繁的研究

徐凌飞,庞 勇,周连霞,王贵章,王 婧,叶红霞   

  • 出版日期:2005-05-05 发布日期:2005-05-05

Micropropagation of Lilium davidii var. unicolor

Xu Lingfei, Pang Yong, Zhou Lianxia, Wang Guizhang, Wang Jing, Ye Hongxia   

  • Online:2005-05-05 Published:2005-05-05

摘要： 以兰州百合的鳞茎增殖为基础，进行了离体微繁研究。鳞茎增殖的适宜激素组合为0.9 mg/L BA +0.1~0.2 mg/L NAA。培养基中总离子浓度和氮浓度过高或过低都会影响兰州百合增殖，3种基本培养基N6、MS、和1/2MS以N6增殖效果最好。适当提高糖浓度对鳞茎增殖有促进作用，在各处理中以蔗糖浓度为40 g/L增殖倍数高。兰州百合的适宜生根培养基为1/2MS+IBA0.1 mg/L，生根率达100%，生根苗移栽成活率达90%。

Abstract: An in vitro propagation protocol based on bulb proliferation has been developed for Lilium davidii var. unicolor . BA 0.9 mg/L + NAA 0.1~0.2 mg/L was suitable for bulb proliferation .It seemed that high or low total ion and nitrogen concentration affected bulb proliferation. N6 was optimal basal medium for bulb proliferation. Improving sugars concentration enhanced the multiplication efficiency. The multiplication efficiency of 40 g/L Sucrose was higher than others. The best rooting response was obtained on 1/2 MS medium supplemented with 0.1 mg/L IBA, on this medium; the rooting frequency was 100%. The survival percentage was 90% when rooting plantlets were transplanted in perlite and soil.