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中国农学通报

中国农学通报 ›› 2006, Vol. 22 ›› Issue (1): 4-4.

所属专题： 生物技术

• 目次 • 上一篇    下一篇

内毒素对肠黏膜微血管内皮细胞内分泌一氧化氮和内皮素的影响

王 新,谭建华,刘金玲,韦旭斌,穆 祥   

  • 出版日期:2006-01-05 发布日期:2006-01-05

The Respective Effects of LPS on the Intestinal Mucosa Microvascular Endothelial Cells Secreting NO and ET

Wang Xin, Tan Jianhua, Liu Jinling, Wei Xubin, Mu Xiang   

  • Online:2006-01-05 Published:2006-01-05

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摘要： 目的是研究内毒素（lipopolysaccharide; LPS）导致肠黏膜微血管内皮细胞损伤的作用机制。将所培养的肠黏膜微血管内皮细胞分为空白对照组和LPS组，每组按时间分为4个亚组：3h、6h、9h、12h。空白对照组加维持培养液，LPS组加1μg/ml LPS培养液，在CO2培养箱内静置培养。结果表明，一氧化氮（NO）分泌明显升高，3h后达到高峰，随后逐渐降低，而内皮素（endothelin; ET）分泌急剧升高，9h后达到高峰，随后又开始逐渐降低，但一直保持在较高的水平。所以引起了NO和ET的升高可能是LPS导致肠黏膜微血管内皮细胞的损伤机制。

Abstract: The aim: To research on the functional mechanism of LPS damaging intestinal mucosa microvascular endothelial cells. The way: Divide the intestinal mucosa microvascular endothelial cells fostered into blank control group and LPS group and further divide each group into four minor groups according to the time: 3h, 6h, 9h, 12h. Add feeding medium into the blank control group while fill 1μg/mlLPS nutrient liquid into LPS group. After that, put them into CO2 incubators and cultivate them statically. Result: The secretion of NO rises obviously and arrives at the peak in three hours before it lowers gradually. The secretion of ET rises rapidly and arrives at the peak in 9 hours, then it begins to lower gradually, however, it always stays at a high level. Conclusion: the functional mechanism of LPS damaging intestinal mucosa microvascular endothelial cells may be that LPS leads to the rise in the secretion of NO and ET.