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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (24): 103-108.

• 农学 农业基础科学 • 上一篇    下一篇

小对叶植株再生及遗传转化体系构建

侯 雨 吴丽爽 王晓萍   

  • 收稿日期:2010-07-27 修回日期:2010-09-26 出版日期:2010-12-20 发布日期:2010-12-20
  • 基金资助:

    哈尔滨师范大学骨干教师资助计划

Regeneration and Genetic Transformation of Aquatic Plants Bacopa monnieri

  • Received:2010-07-27 Revised:2010-09-26 Online:2010-12-20 Published:2010-12-20

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摘要：

为了利用生物技术方法开发及改良水生植物小对叶，以小对叶（Bacopa monnieri）叶片为外植体，建立其组织培养再生体系及根癌农杆菌介导的遗传转化体系。结果表明：小对叶叶盘最佳愈伤组织诱导培养基是MS+6-BA 0.5 mg/L+NAA 0.02 mg/L；最佳分化培养基是MS+6-BA 0.5 mg/L+NAA 0.05 mg/L；最佳生根培养基是1/2MS+IBA 0.1 mg/L。最佳的根癌农杆菌介导的小对叶遗传转化条件是：外植体预培养2天，侵染菌液OD600为0.5，侵染时间7 min、共培养4天和延迟筛选4天，可获得最高转化率21.4%。

关键词: 香蕉 低温害等级 最低气温 相关分析 低温害指标, 香蕉 低温害等级 最低气温 相关分析 低温害指标

Abstract:

In order to explore and improve the aquatic plant Bacopa monnieri with biotechnological methods, regeneration from tissue culture and Agrobacterium-mediated genetic transformation system were established with leaves of Bacopa monnieri as explants. The results showed that the optimal callus induction medium was MS+6- BA 0.5 mg /L+NAA 0.02 mg/L; differentiation medium was MS+6-BA 0.5 mg/L+NAA 0.05 mg/L; rooting medium was 1/2MS+IBA 0.1 mg/L. The best Agrobacterium tumefaciens-mediated genetic transformation conditions were as follows, explants pre-culturing for 2 day, OD600 of infecting bacilli 0.5, infection for 7 min, co-culturing for 4 day and delay screening after 4 day. The highest frequency of transformation occurred in 21.4%.