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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (6): 133-137.

所属专题： 生物技术； 园艺

• 林学 园艺 园林 • 上一篇    下一篇

高温胁迫下外源水杨酸对韭菜硝酸盐累积及PSⅡ光能吸收利用的影响

霍 捷 高志奎 王俊玲 赵 飞   

  • 收稿日期:2010-09-20 修回日期:2010-11-16 出版日期:2011-03-20 发布日期:2011-03-20
  • 基金资助:

    外源水杨酸降低韭菜硝酸盐累积的调控机制研究

Effects of Exogenous Salicylic Acid on Nitrate Accumulation and Light Absorption and Utilization of PhotosystemⅡ in the Leaves of Chinese Chive under High Temperature Stress

  • Received:2010-09-20 Revised:2010-11-16 Online:2011-03-20 Published:2011-03-20

摘要：

本研究以韭菜为试材，研究了外源水杨酸（Salicylic acid, SA）对高温胁迫下韭菜硝酸盐含量及PSⅡ光能吸收利用的影响。结果表明，高温胁迫会抑制韭菜叶片中氮代谢关键酶硝酸还原酶（Nitrate reductase，NR）活性，造成韭菜叶片硝酸盐累积加剧。而高温胁迫下喷施SA，韭菜叶片NR活性显著升高，硝酸盐含量明显降低；叶绿素含量、PSⅡ原初光化学效率[Fv/Fm和Y(Ⅱ)]、光化学淬灭系数（qP）、PSⅡ电子传递能力（Q和ETRmax）均显著提高，非光化学淬灭系数（NPQ）显著下降。由此证明，高温胁迫下外源SA能够促进硝态氮的还原同化，同时减轻高温胁迫对PSⅡ反应中心造成的伤害，保持韭菜叶片较高的光能吸收利用效率，从而保证硝酸盐还原同化过程的顺利进行。

关键词: 近红外光谱分析技术, 近红外光谱分析技术

Abstract:

Effects of exogenous salicylic acid (SA) on nitrate accumulation and light absorption and utilization of photosystem Ⅱ (PSⅡ) in the leaves of Chinese chive under high temperature stress were studied. The activity of nitrate reductase (NR) in nitrogenous metabolism was depressed under high temperature and the nitrate content was increased. The activity of NR was significantly increased and the nitrate content was decreased obviously by spraying exogenous SA than comparison. At the same time, the chlorophyll content, PSⅡ maximal photochemical (Fv/Fm), PSⅡ effective quantum yield [Y(Ⅱ)], photochemical quenching (qP), original quantum efficiencies (Q) and maximum electron transfer rate (ETRmax) significantly increased, but nonphotochemical quenching (NPQ) decreased obviously. Therefore, exogenous SA showed a positive role in reduction and assimilation of nitrate nitrogen, meanwhile it relieved the damage in the photosynthetic system under high temperature stress, improved the light use efficiency of the Chinese chive’s leave, in order to assure the process of nitrate assimilation smooth going.

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