关键词: 农村实用人才, 农村实用人才

Abstract: Abstract: In order to select the proper extraction method of genome DNA from scarab suitable for RAPD –PCR, genome DNA of Holotrichia titanis Reitter was extracted by Improved SDS, Balance Phenol, Lysis Solution and CTAB Method in the laboratory of Qingdao Agriculture University in 2009, and the most proper extraction method was selected by observation on the character of DNA deposition, production & quality analyzing and RAPD-PCR. The results showed that the genome DNA extracted by Improved SDS Method was higher in production, but lower in purity, degraded seriously, and could generate seriously dispersed RAPD –PCR fingerprinting when amplifying with it as template. Genome DNA extracted by Lysis Solution Method was higher in purity, degraded slightly, but lowest in production, and could generate seriously dispersed and nontypical RAPD –PCR fingerprinting when amplifying with it as template. Genome DNA extracted by CTAB Method was not only lower in purity and production, degraded seriously, but also could not produce stable RAPD –PCR fingerprinting when using it as template, and there was nontypical amplification too. Genome DNA extracted by Balance Phenol Method was higher in purity and production, degraded slightly, and could produce stable RAPD –PCR fingerprinting without dispersion when using it as template. So among above four methods, Balance Phenol Method is the most suitable method to extract genome DNA from scarab, which is to be used for PAPD-PCR, Lysis Solution and Improved SDS Method take the second place, and CTAB Method is the most ill-fitted one.