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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (22): 79-83.doi: 10.11924/j.issn.1000-6850.2014-0397

• 林学 园艺 园林 • 上一篇    下一篇

狭叶四照花茎段的离体培养与植株再生

唐佳佳   

  • 收稿日期:2014-02-20 修回日期:2014-04-16 出版日期:2014-08-05 发布日期:2014-08-05
  • 基金资助:
    “948”国家林业局引进项目“观赏性北美四照花种质资源及繁殖技术引进”(2012-04-60)；江苏高校优势学科建设工程；江苏高校协同创新中心资助项目。

In vitro Culture and Plant Regeneration from Stem Segments of Dendrobenthamia angustata

  • Received:2014-02-20 Revised:2014-04-16 Online:2014-08-05 Published:2014-08-05

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摘要： 建立高效的狭叶四照花组培快繁体系。以狭叶四照花茎段腋芽为外植体，研究基本培养基和植物生长调节剂对不定芽诱导、不定芽增殖及生根诱导的影响。不定芽诱导过程中，9个组合处理对不定芽诱导均有促进作用，其中WPM+1.0 mg/L 6-BA+0.05 mg/L KT+0.1 mg/L NAA为最佳组合，萌发率达到86.29%；最适宜不定芽增殖培养基为WPM+1.0 mg/L 6-BA +0.2 mg/L NAA，增殖系数4.07；最适宜芽苗生根培养基WPM+1.0 mg/L IBA，生根率达78.94%，生长状态优于其他处理。本实验建立的组培快繁体系，适合狭叶四照花的植株再生。

关键词: 土壤养分流失, 土壤养分流失

Abstract: The study aims to establish an efficient tissue culture system for rapid propagation of Dendrobenthamia angustata. Using stem segments of Dendrobenthamia angustata as explants, effects of the basic culture medium, different types and the concentrations of plant growth regulators on the germination of axillary buds, proliferation of adventitious bud and rooting induction were examined. All nine hormone combinations promoted germination of axillary buds to some extent and the most suitable hormone combination was WPM + 1.0 mg/L 6- BA + 0.05 mg/L KT + 0.1 mg/L NAA, the germination rate achieved 86.29% at the aforesaid conditions. The combination of WPM+1.0 mg/L 6-BA+0.2 mg/L NAA was the best treatment for the culture of shoot induction, and the multiplication coefficient could reach 4.07. The treatment of WPM+1.0 mg/L IBA had the best effect on rooting induction, and its rooting rate was 78.94% with superior growth performance. The system of organization culture was suitable for regeneration of Dendrobenthamia angustata.