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中国农学通报

中国农学通报 ›› 2017, Vol. 33 ›› Issue (4): 98-104.doi: 10.11924/j.issn.1000-6850.casb16060071

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

丛枝菌根真菌对丹参木质部结构及防御相关基因的影响

周修腾,王 雪,黄璐琦,陈 敏,肖文娟,杨光,李鹏英,华国栋,陈美兰   

  1. 中国中医科学院中药研究所,中国中医科学院中药研究所,中国中医科学院中药资源中心,中国中医科学院中药研究所,郫县中医医院,中国中医科学院中药研究所,中国中医科学院中药研究所,北京中医药大学东方医院药学部,中国中医科学院中药研究所
  • 收稿日期:2016-06-14 修回日期:2017-01-17 接受日期:2016-08-25 出版日期:2017-02-10 发布日期:2017-02-10
  • 通讯作者: 陈美兰
  • 基金资助:
    国家中医药局行业科研专项经费“30 项中药材生产使用技术规范化及其适用性研究”(201407005)

Effects of arbuscular mycorrhizal fungi on the structure of xylem and expression of defense-related genes in Salvia miltiorrhiza

黄璐琦,陈 敏,肖文娟,杨光,李鹏英,华国栋 and 陈美兰   

  • Received:2016-06-14 Revised:2017-01-17 Accepted:2016-08-25 Online:2017-02-10 Published:2017-02-10

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摘要： 旨在初步探讨丛枝菌根真菌GV对丹参木质结构及合成相关基因PAL、C4H,防御相关基因PR1、WRKY的影响。实验设定对照组和菌根组,测定生长90d后菌根对丹参生长情况及木质部结构的影响,同时采用实时荧光定量的方法测定相关基因PAL、C4H及不同时期PR1、WRKY的表达情况。接种菌根90d丹参根部侵染率为90%,基本达到最大值；地上部分鲜重和叶片数显著高于对照组,约为对照组的2.7倍和1.96倍；根部横切部分的木质部面积,积分光密度显著大于对照组；与对照组相比,地下部分木质素合成基因PAL、C4H表达显著提高,地上部分显著降低；丹参防御基因PR1(地下部分)和WRKY表达量在75d前显著增加,地下部分WRKY表达量在130d显著增加。丛枝菌根真菌能促进丹参生长,增加丹参根部木质部面积以及密度,对提高丹参抗性有积极作用。

关键词: 蓖麻籽, 蓖麻籽, 侧向压力系数, 理论公式, 直筒式压榨

Abstract: To study the effects of GV, a arbuscular mycorrhizal fungi, on the structure of xylem and expression of PAL and C4H, two lignin synthesis-related genes and expression of PR1 and WRKY the defense-related genes in Salvia miltiorrhiza. Detecting the effects of GV on the growth and xylem structure of S. miltiorrhiza after treatment of GV for 90 d by comparing the control and GV group, and the expression level of PAL, C4H and PR1, WRKY in different period of time using Real-time PCR. Infection rate of GV on S. miltiorrhiza root reached peak (90 %) in the 90th d; fresh weight of overground part and number of leaves were significantly higher in the GV group, naming 2.7 and 1.96 fold respectively of that of the control; integral optical density and area of xylem in root transection of GV group were significantly greater than that of control; expression level of PAL and C4H in the underground part significantly increased in the GV group, while markedly decreased in the overground part; expression of PR1 in underground part and WRKY in both over and underground part increased significantly before 75 d, and that of WRKY in underground part saw another notable increase in 130 d. Arbuscular mycorrhizal fungi could promote growth, increase the area and density of xylem in the root of S. miltiorrhiza, and play a positive role in its pathogen resistance.

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