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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (8): 194-198.

• 林学 园艺 园林 • 上一篇    下一篇

红叶李与安哥诺李及其杂交子代的RAPD分析

李 单 孟庆瑞 沙翠芸 刘 冰 李盼华 李彦慧   

  • 收稿日期:2010-11-08 修回日期:2010-12-23 出版日期:2011-04-20 发布日期:2011-04-20
  • 基金资助:

    河北省自然科学基金资助项目

The RAPD Analysis of P. salicina×P. atropurupurea and Prunus salicina cv. ‘angenuo’ and Its Hybrids

  • Received:2010-11-08 Revised:2010-12-23 Online:2011-04-20 Published:2011-04-20

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摘要：

利用随机扩增多态性DNA分子标记（RAPD）技术对红叶李与安哥诺李及其杂交子代中的10株植株进行遗传多样性分析。从100个随机引物中筛选出15个引物，然后对所有子代进行分析。利用PopGene3.2软件分析所得图谱的多态位点数、多态位点百分率、Shannon信息指数(I)、Nei's基因多样性指数(H)、遗传距离和遗传一致度。用DPSv2.0软件依据所得到的遗传距离，按照非加权平均距离法（UPGMA）构建树状图。结果表明：15个随机引物共扩增出多态条带89个，总的多态位点百分率为93.54%，平均Shannon信息指数为0.4298，平均Nei's基因多样性指数为0.3574，说明杂交后代具有较丰富的遗传多样性。从树状图可以看出杂交子代的遗传变异较丰富，其中1号植株与母本亲缘关系最近，最有可能稳定遗传母本的红叶性状。

关键词: 药效试验, 药效试验

Abstract:

The random amplified polymorphic DNA (RAPD) markers technique was used to analyze the genetic diversity among P. salicina×P. Atropurupurea and Prunus salicina cv. ‘angenuo’ and 10 hybrids. 15 primers were screened from 100 random primers, and they were amplified in all hybrids. The polymorphic bands, the percentage of polymorphic loci, Shannon information index (I), Nei's genetic diversity index (H), genetic distance and genetic identity were analyzed by using PopGene3.2 software. The dendrogram based on genetic distance was constructed by using DPSv2.0 software, in accordance with the un-weighted pai-group method using an arithmetic average (UPGMA). The results showed that 15 primers amplified 89 polymorphic bands, the total percentage of polymorphic loci was 93.54%, the average Shannon information index was 0.4298, and the average Nei's genetic diversity index was 0.3574, which indicated more genetic diversity of offspring. The dendrogram showed the genetic variation of hybrids was rich. The No.1 had the closest relationship with the parent plant, which indicated it could inherit the red leaves traits of the female parent likely.