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中国农学通报 ›› 2011, Vol. 27 ›› Issue (7): 348-351.

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

夹竹桃叶对蟾蜍离体坐骨神经干动作电位的影响研究

向德标 伍松柏   

  • 收稿日期:2010-11-08 修回日期:2010-12-18 出版日期:2011-04-05 发布日期:2011-04-05

Effects of Nerium indicum Leaves on Compound Action Potential of Sciatic Nerves of Toad

  • Received:2010-11-08 Revised:2010-12-18 Online:2011-04-05 Published:2011-04-05

摘要:

为了研究夹竹桃叶水提取物对蟾蜍离体坐骨神经动作电位的影响,将蟾蜍离体坐骨神经分为三组,分别用10%、20%和40%的夹竹桃水提取物对其进行浸泡,通过BL-420F生物机能实验系统测定各组离体坐骨神经干动作电位的振幅、传导速度、阈强度及不应期。结果显示经不同浓度夹竹桃水提取物处理后,都可引起神经干动作电位振幅减小(p<0.05),传导速度减慢(p<0.05),不应期延长(p<0.05),阈强度增大(p<0.05),并最终使坐骨神经动作电位消失,且动作电位均能恢复。试验结果表明夹竹桃叶水提取物能可逆地阻滞神经动作电位的传导。

关键词: 分子标记技术的新分类, 分子标记技术的新分类

Abstract:

Abstract:To research the effects of the water extract from Nerium indicun leaves on action potential of the toad’s sciatic nerves. The toad’s sciatic nerves were divided into three groups, and infiltration with concentration of 10%,20%and 40%of the water extract from Nerium indicum leaves. the amplitude, conduction velocity, threshold intensity and refractory period of action potentials of the sciatic nerves from each group were measured respectively by BL-420F organism function experiment system. The conclusion of study had indicated that the water extract from Nerium indicun leaves could decrease the conduction velocity (p<0.05),slowed down the conduction velocity (p<0.05),extended the refractory period (p<0.05),and increase the threshold intensity(p<0.05).The action potential of sciatic nerves vanished at last. The action potential of sciatic nerves could recover. The conclusion of study had indicated that the water extract from Nerium indicum leaves could block the conduction of action potential of nerve reversibly.