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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (12): 91-95.

所属专题： 油料作物； 烟草种植与生产

• 农学 农业基础科学 • 上一篇    下一篇

大豆GmMYB12B2植物表达载体的构建及转化烟草

李晓薇 张艳 赵旭 钱丹丹 闫帆 张庆林 李景文 王庆钰   

  • 收稿日期:2010-12-01 修回日期:2010-12-23 出版日期:2011-05-25 发布日期:2011-05-25
  • 基金资助:

    转基因生物新品种培育重大专项子课题;国家自然科学基金面上项目;吉林省科技发展计划重点项目;长春市科技局国际科技合作项目

Construction of Plant Expression Vector of Soybean GmMYB12B2 Gene and Transformation in Tobacco

  • Received:2010-12-01 Revised:2010-12-23 Online:2011-05-25 Published:2011-05-25

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摘要：

为研究大豆MYB转录因子—GmMYB12B2在转基因植物类黄酮代谢中的作用，利用吉林大学植物种质资源与利用研究室前期克隆的GmMYB12B2基因，以PPZP质粒为表达载体，构建了由组成型CaMV35S启动子驱动GmMYB12B2基因的植物表达载体PPZP-GmMYB12B2；然后，采用冻融法转入农杆菌EHA105菌株，通过叶盘法对烟草进行遗传转化。经PCR随机扩增鉴定9株转基因烟草，其中有6株是阳性植株，初步证明已获得转大豆GmMYB12B2基因的烟草。

关键词: 数据采集, 数据采集

Abstract:

In order to study the application of GmMYB12B2 gene in transgenic plants involving flavonoids metabolism, the plant expression vector PPZP-GmMYB12B2 was constructed, in which the GmMYB12B2gene was driven by the constitutive promoter CaMV35S. The constructed expression vector was transformed into EHA105 strain by freeze -thaw method. Leaf segments of tobacco were inoculated with EHA105 containing plasmid PPZP-GmMYB12B2. The transgenic lines were selected by PCR detection, in nine random transgenic tobaccos, six of them were positive plants. The result showed that GmMYB12B2 gene had been integrated into the genome of tobacco.