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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (12): 201-204.

所属专题： 玉米

• 农学 农业基础科学 • 上一篇    下一篇

利用SSR标记技术鉴定玉米品种真实性的研究

岳静 朱志成 申雅娟 张卉 王汝宝   

  • 收稿日期:2010-12-17 修回日期:2011-02-09 出版日期:2011-05-25 发布日期:2011-05-25

Study on Identifying the Authenticity of Maize Varieties by SSR Markers

  • Received:2010-12-17 Revised:2011-02-09 Online:2011-05-25 Published:2011-05-25

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摘要：

从20对SSR基本引物中筛选出核心引物，并对辽宁省主推玉米品种进行真实性研究。利用SSR标记技术，选取均匀覆盖玉米染色体组的20对SSR引物对玉米品种进行PCR扩增分析。依据带型清晰与否、稳定性等筛选出14对核心引物用于辽宁省主推玉米品种的真实性鉴定，其中以引物bnlg161k8的多态性最好，且仅采用bnlg161k8、bnlg2305k4及umc1705w1三对引物即可以对实验中所选玉米品种进行真实性鉴定。bnlg161k8、bnlg2305k4及umc1705w1这三对引物可作为玉米品种真实性鉴定的首选引物，既节约成本，又提高效率。

关键词: 常温贮藏, 常温贮藏

Abstract:

In this study, the core primers were selected from 20 pairs of SSR primers to identify the authenticity of maize varieties in Liaoning province. PCR amplified products of 10 maize hybrids were detected by 20 basic primer pairs averagely distributed on 10 chromosomes of maize by using SSR markers. 14 pairs of core primers were selected to identify the authenticity of maize varieties on electrophoresis maps and the stability,et al.Among all the core primer pairs, bnlg161k8 was the best on polymorphism and the authenticity of 10 maize varieties was identified only by 3 pairs of primers (bnlg161k8, bnlg2305k4, umc1705w1). 3 pairs of primers (bnlg161k8, bnlg2305k4, umc1705w1) could be the preferred primers by costing savings and improving efficiency.