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中国农学通报 ›› 2011, Vol. 27 ›› Issue (11): 10-14.

所属专题: 生物技术 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

秦川牛微卫星基因位点遗传多样性分析

王洪程 昝林森 宋付标   

  • 收稿日期:2010-12-20 修回日期:2011-01-31 出版日期:2011-05-15 发布日期:2011-05-15
  • 基金资助:

    国家863计划;国家“十一五”科技支撑计划;教育部“长江学者和创新团队发展计划”

The analysis of genetic diversity of microsatellite loci in Qinchuan Cattle

  • Received:2010-12-20 Revised:2011-01-31 Online:2011-05-15 Published:2011-05-15

摘要:

利用PCR 技术和电泳银染技术检测了秦川牛BM2113、IDVGA2、TGLA44、IDVGA27、ETH10、ILSTS005、和IDVGA46等7个微卫星位点的多态性分布,计算了各个基因座的表观及期望杂合度、有效等位基因数、多态信息含量、固定指数和Shannon信息熵。结果显示,秦秦川牛群体的遗传杂合度较高,遗传多样性丰富,所选微卫星位点可用于秦川牛遗传多样性评估。

关键词: 副猪嗜血杆菌, 副猪嗜血杆菌, 高免血清, 琼扩试验, 血清分型

Abstract:

The polymorphism distributions of microsatellite loci , BM2113、IDVGA2、TGLA44、IDVGA27、ETH10、ILSTS005、and IDVGA46 were detected in Qinchuan cattle by Polymerase Chain Reaction and multiplex gel electrophoresis followed by silver staining . The results indicated that genetic heterozygosity of Qinchuan cattle was high,genetic diversity Was rich.The selected microsatellite loci can be used to evaluate genetic diversity of Qinchuan cattle.