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中国农学通报 ›› 2016, Vol. 32 ›› Issue (2): 1-4.doi: 10.11924/j.issn.1000-6850.casb15070129

所属专题: 生物技术

• 畜牧 动物医学 蚕 蜂 •    下一篇

副猪嗜血杆菌中毒素蛋白基因hipA的生物信息学分析

车勇良,陈如敬,王隆柏,吴学敏,刘玉涛,王晨燕,周伦江   

  1. (福建省农业科学院畜牧兽医研究所/福建省畜禽疫病防治工程技术研究中心,福州 350013)
  • 收稿日期:2015-07-22 修回日期:2015-11-26 接受日期:2015-12-03 出版日期:2016-01-28 发布日期:2016-01-28
  • 通讯作者: 周伦江
  • 基金资助:
    福建省公益类科研专项“副猪嗜血杆菌病的流行病学调查与诊断技术研究”(2010R1025-2),福建省农科院畜牧兽医研究所所青年基金“副猪嗜血杆菌hipBA基因缺失株的构建及其生物学功能研究”(MYQJ2014-4)。

Bioinformatic Analysis of Toxin Protein Gene hipA from Haemophilus parasuis

Che Yongliang, Chen Rujing, Wang Longbai, Wu Xuemin, Liu Yutao, Wang Chenyan, Zhou Lunjiang   

  1. (Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences/Fujian Animal Diseases Control Technology Development Center, Fuzhou 350013)
  • Received:2015-07-22 Revised:2015-11-26 Accepted:2015-12-03 Online:2016-01-28 Published:2016-01-28

摘要: 为预测毒素蛋白HipA的二级结构和B细胞抗原表位,首先从GenBank中查找副猪嗜血杆菌毒素蛋白基因hipA的序列,应用生物信息学方法,对副猪嗜血杆菌毒素蛋白HipA的理化性质、二级结构和B细胞抗原表位进行预测。结果显示,HipA蛋白的理论等电点为7.23,前50个氨基酸为信号肽,二级结构的预测结果显示该酶主要以α螺旋、无规则卷曲和延伸链为主,只有少数的β转角;推测该蛋白有17个B细胞优势抗原表位区域。该研究为进一步研究分析副猪嗜血杆菌毒素蛋白HipA的功能奠定理论基础。

关键词: 鱼腥草, 鱼腥草, 草鱼, 生长性能, 生理生化指标

Abstract: The aim of this study was to predict secondary structure and B-cell epitopes of toxin protein HipA of Haemophilus parasuis. The hipA gene sequence was searched from GenBank. By using bioinformatics methods, the physicochemical property, the secondary structure and B-cell epitope of HipA protein were predicted. The results showed that the IP of HipA was 7.23 and the first 50 amino acids was signal peptide. Using the SOPMA method, prediction of the secondary structure of the HipA showed that α-helix, random coils and extended strand were the main structural types of the flexible region in secondary structure, and there was a small amount of β-turn. The HipA protein was supposed to contain 17 potential antigen epitopes. These results provided a theoretical basis for a further study of the toxin protein (HipA) from Haemophilus parasuis.

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