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中国农学通报

中国农学通报 ›› 2004, Vol. 20 ›› Issue (4): 121-121.

所属专题： 马铃薯

• 目次 • 上一篇    下一篇

外植体对甘薯茎尖培养与植株再生的影响

唐 君,赵冬兰,刘亚菊   

  • 出版日期:2004-08-05 发布日期:2004-08-05

EFFECT OF EXPLANT ON SWEETPOTATO MERISTEM CULTURE AND PLANTLET REGENERATION

TANG JUN, ZHAO Dong – lan, LIU Ya – ju   

  • Online:2004-08-05 Published:2004-08-05

摘要： 对甘薯茎尖外植体的取材大小，不同取材季节，不同消毒处理及不同生育时期的试验结果表明，外植体的大小对其茎尖离体培养的成苗率及脱毒率有显著差异，尤其是取材的季节和不同生育时期，对甘薯茎尖培养的效果具有明显的差异，本试验以1～4月份取薯块催芽的外植体材料是甘薯茎尖培养较为适宜的培养时间，其污染、死亡率为15%；茎尖成活率85%以上；品种成苗率可达86%。

Abstract: The Sweetpotato(I pomoea batatas lam,) meristem culture and plantlet regeneration were studied, using different explants, different sterile methods and different growth stages.The results showed that the sizes of explants is important for plantlet rations and virus free rations, expecially the season and the growth stage of materials. From January to April were feasible time to take the sweetpotato roots for accelerating germination, and the explants were more feasible for cristem culture. The rate of ecesis of cristem was more than 85% and variety plantlet ration could reach 86%.