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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (10): 260-265.

所属专题： 生物技术； 园艺

• 林学 园艺 园林 • 上一篇    下一篇

草莓ABA结合蛋白基因的克隆及序列分析

刘雅萍 张希 葛安静 董清华   

  • 收稿日期:2011-01-05 修回日期:2011-02-21 出版日期:2011-05-15 发布日期:2011-05-15
  • 基金资助:

    北京市教委和基金委联合重点项目

Cloning and Sequencing of CHLH Gene in Fragaria pentaphylla Lozinsk

  • Received:2011-01-05 Revised:2011-02-21 Online:2011-05-15 Published:2011-05-15

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摘要：

为进一步研究ABA结合蛋白在草莓抗逆性方面的作用，本文通过CTAB法提取五叶草莓总DNA，根据NCBI数据库中的ABA结合蛋白受体的基因序列信息，设计特异引物，克隆得到4426 bp的ABA结合蛋白基因。序列分析表明该基因全长4662 bp，编码1393个氨基酸，核苷酸序列比对表明，该序列与GenBank中其他物种的CHLH序列有极高的同源性，核苷酸序列比较相似率在77%~90%，氨基酸相似性在63%~73%。

关键词: 总有机碳, 总有机碳

Abstract:

The aim was to study the functions of abscisic acid binding protein in straw berry resistance. Therefore, the research of abscisic acid binding protein was the crux of the problem in the mechanism of ABA. In this study, the total DNA was extracted from Fragaria pentaphylla Lozinsk leaves, and the cDNA sequence was amplified through PCR. The results showed gene had 4662 bp in length, encoding 1393 amino acid. The CHLH has high sequence homology with other plants，it shared 77%-90%identity in nucleotide sequence and 57%-77% identity in amino acid sequence.