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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (10): 256-259.

所属专题： 生物技术； 园艺

• 林学 园艺 园林 • 上一篇    下一篇

葡萄病毒AMP基因RNA干扰载体的构建

丁艳杰 周宗山 董雅凤 徐成楠 吴玉星 迟福梅   

  • 收稿日期:2011-01-12 修回日期:2011-02-23 出版日期:2011-05-15 发布日期:2011-05-15
  • 基金资助:

    现代农业产业技术体系专项资金资助;农业部公益性行业科研专项

Construction of the RNAi Vector of GVA MP Gene

  • Received:2011-01-12 Revised:2011-02-23 Online:2011-05-15 Published:2011-05-15

PDF (PC)

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摘要：

RNA干扰（RNAi）介导的植物抗病毒研究是近年来引起广泛关注的一项植物抗病毒基因工程策略。本文以GVA运动蛋白（MP）基因为模板扩增出了418 bp的基因片段，根据RNA干扰的基本原理，将目的片段正反向分别插入到载体pFGC5941内含子的两侧以便其转录过程中高效产生RNA发夹结构。经PCR扩增证明已成功构建以GVA MP基因为靶标的干扰载体pFGC5941-GVAFR，并成功转入农杆菌EHA105中，为后续探讨干扰载体的干涉效果奠定了基础。

关键词: 水稻 4001 多芽 突变体, 水稻 4001 多芽 突变体

Abstract:

The resistance induced by RNAi to plant virus is a new strategy for controlling the viruses in recent years. In this study, according to the basic principles of RNA interference, two fragments (418 bp) of GVA MP gene were amplified with PCR and inserted into both sides of intron of pFGC5941 in forwards and reverse way so that it produces hairpin RNA during translation efficiently. The construction of the interfering vector pFGC5941-GVAFR targeting to GVA MP gene was identified by PCR, and the vector was transferred into agrobacterium EHA105 successfully, it provided an important basis for further studying its RNAi effection.

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