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中国农学通报 ›› 2011, Vol. 27 ›› Issue (9): 176-179.

所属专题: 生物技术 油料作物 园艺

• 农学 农业基础科学 • 上一篇    下一篇

甘蓝型油菜雄性不育系09A花蕾发育过程中生理生化特性研究

王瑞雪 沈亮余 邹燕 张涛 龚慧明   

  • 收稿日期:2011-01-19 修回日期:2011-02-17 出版日期:2011-04-25 发布日期:2011-04-25

Studies on the Physiological and Biochemical Characters in Developing Bud of Male Sterile line 09A of Brassica napus

  • Received:2011-01-19 Revised:2011-02-17 Online:2011-04-25 Published:2011-04-25

摘要:

通过分光光度法,对油菜细胞质雄性不育系09A和保持系09B不同发育时期花蕾中POD、CAT、SOD、APX的活性和MDA、游离Pro含量进行了测定,并讨论分析了与雄性不育之间的关系。结果表明:不育系09A不同发育阶段花蕾中CAT、SOD、APX三种酶活性均低于09B;POD活性在不育系花蕾发育早期低于09B,但随着花蕾的发育,09B的POD活性逐渐下降,而09A的POD活性则逐渐升高;不育系09A的MDA含量在整个花蕾发育过程中均高于09B,而Pro含量则低于09B。

关键词: 村镇建设, 村镇建设

Abstract:

The enzyme activities of the rapeseed cytoplasmic male sterile lines of 09A and fertile lines of 09B , including peroxidase(POD), catalase(CAT), superoxide dismutase(SOD) and ascorbic acid peroxidase(APX) of different development stages of bud, were assayed by using spectrophotometry, the relationship between four kinds of protective enzyme activity and cytoplasmic infertility were investigated in this paper. At the same time, the content of malondialdehyde (MDA) and free proline were also assayed by spectrophotometry. The results indicated that along with the development of the bud, the enzyme activities of CAT, SOD, and APX were lower than fertile lines. POD activity was lower than fertile lines in the early development of the bud, but with the development of the bud growth to later period, POD activity of fertile lines of 09B gradually declined, and the POD activity of cytoplasmic male sterile was increasing gradually, and higher than 09B at last. In the cytoplasmic male sterile lines 09A, during the development of the bud, the content of the MDA were always higher than 09B, but the content of proline were lower than the fertile lines 09B.