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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (8): 63-68.

• 林学 园艺 园林 • 上一篇    下一篇

入侵植物薇甘菊和三叶鬼针草对土壤细菌群落的影响

陈亮 李会娜 杨民和 万方浩   

  • 收稿日期:2011-02-09 修回日期:2011-02-21 出版日期:2011-04-20 发布日期:2011-04-20
  • 基金资助:

    农林危险生物入侵机理与控制基础研究

The Influence of Invasion of Mikania micrantha and Bidens pilosa to the Bacterial Community in the Root Soils

  • Received:2011-02-09 Revised:2011-02-21 Online:2011-04-20 Published:2011-04-20

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摘要：

【研究目的】通过比较外来植物薇甘菊和三叶鬼针草对入侵地土壤细菌群落的影响，以期揭示外来植物入侵的土壤微生物学机制。【方法】基于16S rDNA的PCR-DGGE图谱分析，结合条带割胶回收DNA进行序列分析的方法，对入侵植物薇甘菊和三叶鬼针草在广州不同入侵程度的土壤中细菌群落的多样性及优势类群进行比较分析。【结果】DGGE图谱分析结果表明：薇甘菊重度入侵与轻度入侵、重度入侵与未入侵样品中的细菌群落结构的相似性分别为59.6%和54.2%；三叶鬼针草重度入侵与轻度入侵、重度入侵与未入侵样品中的细菌群落结构的相似性分别为60.0%和58.6%。DGGE图谱中特异性条带序列分析表明：薇甘菊和三叶鬼针草入侵引起变化的优势菌群为与α、β、γ变形菌纲和芽孢杆菌有很近亲缘关系的不可培养细菌。【结论】2种入侵植物在不同入侵程度土壤中改变了土壤细菌群落的组成和种类，有可能形成有利于自身生长的微环境，以便于进一步扩张。

关键词: 常规品种, 常规品种, 湘早籼46号, 主要特征特性, 产量结构

Abstract:

【OBJECTIVE】Understanding the influence of invasion of Mikania micrantha and Bidens pilosa to the bacterial community in the root soils.【METHOD】The PCR-DGGE combined with cloning of V3 region of bacterial 16S rDNA fragments were used to analyze the bacterial community of the rhizospheric soils, which were heavily invaded, newly invaded or non-invaded by the two exotic weeds, Mikania micrantha and Bidens pilosain in Guangzhou.【RESULTS】The analysis of DGGE profiles showed that: comparing with the non-invaded site, the soil examples of the heavily-invaded and the newly-invaded by Mikania micrantha in Guangzhou had the similarity of 59.6% and 54.2%, respectively; the same trend occurred in the soil samples of the heavily-invaded and the newly invaded by Bidens pilosa, the similarity were 60.0% and 58.6%, respectively. The invasion of Mikania micrantha and Bidens pilosa resulted in the changes of uncultivated bacteria which had close relationships to α, β, γ proteus and bacilli in the soils.【CONCLUSION】All those results suggested that the invasion of Mikania micrantha and Bidens pilosa could modified soil microbial community composition, probably create favorable soil environment to benefit themselves.