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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (29): 33-37.

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

PCV2感染猪皮肤源树突状细胞内源性抗原加工提呈相关分子转录水平的变化

李建东 李焕荣 聂晓华 遇奇 崔德凤   

  • 收稿日期:2011-03-24 修回日期:2011-05-13 出版日期:2011-11-15 发布日期:2011-11-15

Changes of the Transcription Levels of Molecules Associated with Endogenous Antigen Processing and Presentation of Porcine Skin-derived Dendritic Cells Infected with PCV2 in vivo

  • Received:2011-03-24 Revised:2011-05-13 Online:2011-11-15 Published:2011-11-15

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摘要：

用猪圆环病毒2型（PCV2）感染40日龄健康长白仔猪，于感染后3、7、14、21和35天宰杀，收集皮肤源树突状细胞（DC）。利用实时荧光定量PCR技术对感染仔猪皮肤源DC内源性抗原加工提呈相关分子（LMP7，UBP，MHC-I，calreticulin）mRNA水平的变化进行定量分析。结果显示，LMP7转录水平在感染后3天显著下调（P < 0.05），UBP转录水平始终上调，其中在21天和35天显著上调（P < 0.05），MHCⅠ转录水平在7 天显著下调（P < 0.05），calreticulin转录水平虽有所上调，但差异不明显。以上结果表明，PCV2在感染早期可抑制猪皮肤源DC内源性抗原加工提呈能力。

关键词: 雷电 灾害 现状 农村 防御对策, 雷电 灾害 现状 农村 防御对策

Abstract:

Healthy Large White piglets of forty-day-old were euthanized on 3, 7, 14, 21 and 35 days post-infected with porcine circovirus type 2 (PCV2) and their skin-derived dendritic cells (DCs) were collected for analyzing the transcription levels of molecules (LMP7, UBP, MHC-I, calreticulin) associated with endogenous antigen processing and presentation by real-time fluorescent quantitative PCR (real-time FQ-PCR). The results showed that the transcription levels of LMP7 mRNAs were decreased significantly on 3 days post inoculation (DPI) (P < 0.05). UBP and calreticulin mRNAs were consistently higher during the experiment, only with significant differences on 21 and 35 DPI (P < 0.05) for UBP. And MHCⅠwere decreased significantly on 7 DPI (P < 0.05). All of the above results indicated a reduction in the ability of endogenous antigen processing and presentation of porcine skin-derived DCs during the early period of PCV2-infected piglets.