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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (30): 237-240.

所属专题： 生物技术

• 食品 营养 检测 安全 • 上一篇    下一篇

银杏愈伤组织细胞中总黄酮提取工艺的比较

邵菊芳 朱红威 马毅 叶翔 刘炯天   

  • 收稿日期:2011-03-24 修回日期:2011-07-08 出版日期:2011-11-25 发布日期:2011-11-25
  • 基金资助:

    中国矿业大学化学学科理科专项基金

Comparison of Total Flavonoids Extracting Methods on Ginkgo biloba Callus

  • Received:2011-03-24 Revised:2011-07-08 Online:2011-11-25 Published:2011-11-25

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摘要：

为探索从药用植物细胞中提取黄酮类活性物质，用简单、易工业实现的低成本工艺获得较大的总黄酮提取率，为工业生产提供理论依据和实践指导，以银杏愈伤组织为材料提取总黄酮并测定含量。利用乙醇提取、超声波提取和水提取3种方法对银杏愈伤组织中黄酮类化合物进行提取，以络合分光光度法测定的黄酮得率为衡量指标，得到各自最佳的提取条件。结果表明：利用乙醇法提取愈伤组织中总黄酮提取最佳条件是：80%的乙醇作为提取剂，料液比为1∶7（m∶v），于60℃提取3次，总黄酮得率为3.67%；超声波法提取最佳条件为60%的乙醇作为提取剂，料液比为1∶34（m∶v），提取65 min总黄酮，得率为4.57%；水提法的最佳条件是：水温90℃，料液比为1∶30（m∶v），提取3.5 h，总黄酮得率为2.95%。3种优化后的提取方法黄酮得率依次为：超声法＞乙醇法＞水提法。

关键词: 农产品流通 体制变革 动因 趋势, 农产品流通 体制变革 动因 趋势

Abstract:

In order to explore an easily industrialized and lower costs method for flavonoids extraction from medical plants cells, technique was studied to provide theoretical foundations and practical directions for industrial production. Total flavonoids which were from Ginkgo biloba callus were extracted and determined. ethnol extraction, ultrasonic technology and water immersion extraction were used in this experiment. The content of total flavonoids was determined by spectrophotometric methods. The optimal conditions in ethnol extraction can be gotten as follows: ratio of material to solvent in 80% ethanol = 1∶7 (m∶v), temperature 60℃, extracting time thrice and the extracting ratio 3.67%. The optimal conditions in ultrasonic technology were that ratio of material to solvent in 60% ethanol = 1∶34 (m∶v)，the time 65 min and the extracting ratio 4.37%. The optimal conditions in water immersion extraction can be gotten as follows: ratio of material to solvent in water= 1∶30 (m∶v)，temperature 90℃, the time 3.5 h and the extracting ratio 2.95%. The order of flavlnoids extracting ratio in three optimized methods was ultrasonic technology＞ethnol extraction＞water immersion extraction.