欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (20): 133-136.

• 食品 营养 检测 安全 • 上一篇    下一篇

余甘子多糖提取物抗氧化活性研究

高路 张公信 高云涛   

  • 收稿日期:2011-03-25 修回日期:2011-05-09 出版日期:2011-08-15 发布日期:2011-08-15
  • 基金资助:

    云南省社会发展科技计划项目

The Study on the Antioxidant Activity of Polysaccharide Extract from Phyllanthus emblica L.

  • Received:2011-03-25 Revised:2011-05-09 Online:2011-08-15 Published:2011-08-15

PDF (PC)

1201

摘要：

利用超声提取-水提醇沉法从余甘子中提取可溶性多糖，多糖提取率达4.61%。提取物中多糖含量为44.62%。考查了余甘子多糖提取物的抗氧化活性，对Fenton反应羟自由基清除作用的IC50为0.38 mg/mL，最大清除率为80.3%，对光照核黄素超氧阴离子自由基清除作用的IC50为0.93 mg/mL，最大清除率为85.9%，对羟自由基诱发卵磷脂脂质过氧化损伤的IC50为0.39 mg/mL，最大抑制率为71.3%。实验结果表明余甘子富含活性多糖，余甘子多糖具有良好的抗氧化作用，最大清除作用与茶多酚相近，具有较好的研究价值。

关键词: 赤潮, 赤潮

Abstract:

The water soluble polysaccharide was extracted from phyllanthus emblica L. by the method of ultrasound-water extracting-alcohol precipitating. Polysaccharide extraction yield of 4.61% was obtained and the content of polysaccharide in the extract was reached to 44.62%. The antioxidant activity of the polysaccharide of phyllanthus emblica L. was investigated. The IC50 of scavenging effect on hydroxyl free radical generated by Fenton reaction was 0.38 mg/mL, the maximum scavenging rate was 80.3%. The IC50 of scavenging effect on superoxide radical generated by riboflavine photosensitization was 0.93 mg/mL, the maximum scavenging rate was 85.9 %. The IC50 of inhibition effect on lecithin lipid peroxidation induced by hydroxyl free radical was 0.39 mg/mL, the maximum inhibition rate was 71.3%. The results showed that phyllanthus emblica L. polysaccharides had good antioxidant activity, which was closed to tea polyphenols.