欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (16): 151-155.

所属专题： 生物技术； 园艺

• 林学 园艺 园林 • 上一篇    下一篇

南瓜CmNAC基因片段的克隆与序列分析

郝芮 赵福宽 孙清鹏 杨爱珍   

  • 收稿日期:2011-03-28 修回日期:2011-04-18 出版日期:2011-07-05 发布日期:2011-07-05
  • 基金资助:

    北京市教委“植物逆境生物学科研平台建设”项目

Cloning and Sequence Analysis of CmNAC Gene Fragments in Cucurbita moschata

  • Received:2011-03-28 Revised:2011-04-18 Online:2011-07-05 Published:2011-07-05

PDF (PC)

1577

摘要：

NAC转录因子是特异存在于植物中具有多种生物功能的新型转录因子，其家族成员N端含有保守氨基酸序列，C端为高度变异的转录激活区。本项研究以南瓜叶片为材料，根据甘蓝型油菜NAC1、番茄NAC、辣椒NAC保守结构域设计一对简并引物，采用RT-PCR方法扩增得出长度约为440 bp大小的DNA片段，将其克隆至pMDl9-T载体上，而后对重组克隆进行测序，用BLAST和DNAMAN软件对核酸及氨基酸序列进行分析，结果表明所获得的南瓜NAC基因片段由442个碱基组成，编码147个氨基酸，命名为CmNAC。该基因片段具有其他植物NAC基因中存在的保守区，并且属于NAC家族中ATAF1/2亚家族。

关键词: 余甘子 SRAP 优化, 余甘子 SRAP 优化

Abstract:

NAC transcription factors that are unique to plants are the new type transcription regulatory factors with multiple biological functions. Their family proteins contain a conserved amino acid sequence in N-terminal ends and highly different transcription regulatory domain in C terminal ends. In this experiment, a pair of degenerate primers was designed based on the conserved sequences of NAC gene from Brassica napus, tomato and Chili. NAC transcription factor gene fragment about 440 bp was amplified by RT-PCR from Cucurbita moschata leaves and cloned into pMD-19T vector, then the recombinant clones were sequenced. The sequences of nucleic acid and amino acid were analyzed using BLAST and DNAMAN software. The results showed that NAC transcription factor gene gained from Cucurbita moschata included 442 bp, encoding 147 amino acids, which named CmNAC. The NAC gene fragments contain a conserved region like other plant NAC genes and belong to the NAC family ATAF1/2 subfamily.