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中国农学通报 ›› 2011, Vol. 27 ›› Issue (25): 283-287.

• 林学 园艺 园林 • 上一篇    下一篇

粉蕉试管苗工厂化生产中的光温调控

梁钾贤   

  • 收稿日期:2011-04-25 修回日期:2011-07-04 出版日期:2011-10-05 发布日期:2011-10-05
  • 基金资助:

    广东省科技厅农业攻关项目《高产优质黄秆香蕉新品种的选育及繁殖推广》

Control of Temperature and Light Intensity during Test-tube Seedling Production of Musa ABB Pisang Awak

  • Received:2011-04-25 Revised:2011-07-04 Online:2011-10-05 Published:2011-10-05

摘要:

为解决粉蕉试管苗生产过程中丛生芽増殖率低、生根苗根少和植株纤细瘦弱等问题,以粉蕉丛生芽为材料,研究温度对丛生芽増殖和光温对试管苗生长的影响。结果表明,温度对丛生芽增殖影响显著。32℃的芽增殖率最高,芽长和鲜重最大,褐变最轻。阶段变温培养和32℃恒温培养相比,芽増殖倍率显著提高、褐变率显著降低、增殖芽的质量明显提高。不同温度和光照下的生根率均为100%。光照强度、温度和光温互作对试管苗生长影响显著。26℃和500~1250 lx条件下培养更有利于根系生长,在500~2500 lx范围内粉蕉生根苗苗高随光照强度增加而增高,光温互作对粉蕉展叶数影响极显著,1250 lx、26℃培养条件下的展叶数最多。

关键词: 克拉伯水丝蚓, 克拉伯水丝蚓, Cu2+离子, 半致死浓度(LC50), 超氧化物歧化酶(SOD)

Abstract:

The effects of temperature and light intensity on the test-tube seedling production of Musa ABB Pisang Awak were studied in order to solve the problems such as low proliferation efficiency and low seedling quality. The results showed that temperature had significant effects on bud proliferation. The bud proliferation rate was the highest and the bud length was longest, fresh weight was the biggest; the grade of browning was the lowest at 32℃ constant temperature. Bud proliferation rate was significantly increased, browning rate significantly reduced, the quality of the proliferation buds apparently improved when buds incubated at stage variable temperature compared to at 32℃ constant temperature. All rooting rates were 100% under different culture temperature and light. The effects of light intensity, temperature and interaction of light and temperature on tube seedling growth were significant. 26℃ and 500-1250 lx favored root formation and growth; at the range from 500 lx to 1250 lx, the height of seedlings was increased with the increase of light intensity; the interaction effect of high and temperature on leaf number was extremely significant, leaf number of seedling was the highest under 26℃ and 1250 lx light intensity.