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中国农学通报

中国农学通报 ›› 2005, Vol. 21 ›› Issue (4): 44-44.

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Genetic Diversity of Cultivated Chinese Chive Germplasm by ISSR and RAPD Markers

韭菜栽培品种遗传多样性的ISSR和RAPD研究   

  • 出版日期:2005-04-05 发布日期:2005-04-05

Genetic Diversity of Cultivated Chinese Chive Germplasm by ISSR and RAPD Markers

Pan Min, Yang Jianping, Cao Dehang, Li Xincheng, Liu Lianhang, Li Yongxiang   

  • Online:2005-04-05 Published:2005-04-05

摘要： 利用RAPD(Random Amplified Polymorphic DNA)和ISSR(Inter-simple Sequence Repeat)两种分子标记技术对20份韭菜栽培品种进行了遗传多样性研究。结果表明，筛选后选用的12个ISSR引物和15个RAPD引物分析分别产生了258和101条扩增产物带，其中多态性条带(即20个韭菜品种中一个或多个但不是全部具有的带)分别为132和40条，分别占总数的51.2%和39.6%。，也就是说12个ISSR引物和15个SSR引物对韭菜不同品种的扩增可分别产生51

Abstract: Random amplified polymorphic DNA (RAPD) and Inter-simple sequence repeat (ISSR) molecular markers were used to analyze the genetic diversity of 20 cultivated Chinese chive’s germplasm. The results are as fellows: Of the primers tested, 12 ISSR and 15 RAPD