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中国农学通报

中国农学通报 ›› 2005, Vol. 21 ›› Issue (4): 48-48.

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Construction of Recombinant Expression Plasmid pYELmleA of mleA Gene and Expression in Saccharomyces cerevisiae

刘延琳,蒋思欣,何秀萍,李 华,张博润   

  • 出版日期:2005-04-05 发布日期:2005-04-05

Construction of Recombinant Expression Plasmid pYELmleA of mleA Gene and Expression in Saccharomyces cerevisiae

Liu Yanlin, Jiang Sixin, He Xiuping, Li Hua, Zhang Borun   

  • Online:2005-04-05 Published:2005-04-05

摘要： 苹果酸－乳酸酶是进行MLF的功能酶。笔者进行酒酒球菌SD-2a的苹果酸－乳酸酶基因重组表达质粒的构建，利用来自重组质粒pLmleA的mleA基因，以PGK1强启动子和ADH1终止子为调控元件，以大肠杆菌－酵母菌穿梭质粒YEp352为载体，构建了重组表达质粒pYELmleA，并转化酿酒酵母（Saccharomyces cerevisiae）YS58。酵母转化子用含有亮氨酸、组氨酸和色氨酸的YNB平板筛选鉴定。SDS-PAGE检测表明获得的转化子表达了约60KD的目标蛋白，斑点杂交检测表明目的基因mleA转化到

Abstract: Malolactic enzyme is the function enzyme to turn L-malic acid into L-lactic acid during malolactic fermentation (MLF). In this paper, mleA gene encoding for malolactic enzyme from pLmleA, PGK1 promoter from plasmid pVC727-6 and ADH1 terminator from plasmid