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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (31): 184-188.

• 林学 园艺 园林 • 上一篇    下一篇

枣茎段再生体系建立的研究

王国平 肖蓉 李春燕 张拥兵   

  • 收稿日期:2011-05-20 修回日期:2011-08-17 出版日期:2011-12-05 发布日期:2011-12-05
  • 基金资助:

    山西省回国留学人员科研项目

Study on the Establishment of Stem Regenerating System from Ziziphus jujuba

  • Received:2011-05-20 Revised:2011-08-17 Online:2011-12-05 Published:2011-12-05

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摘要：

以‘骏枣’茎段为试验材料，通过组织培养技术的应用，研究影响茎段不定芽再生的主要因素。通过试验，选择出不定芽再生的适宜条件，建立枣茎段的高频再生系统，为基因工程技术在枣上的应用奠定良好基础。试验结果表明：茎段最佳取材阶段为5月中旬；最好的灭菌方法是间歇性灭菌处理；对内生菌抑制效果最好的抗生素是添加480 mg/L青霉素；茎段在培养基中的插入方式以竖直插入培养基方式接种，效果最好；在培养基1/2MS +6-BA 2.0 mg/L + IBA 0.5 mg/L上，不定芽再生效果最好。

关键词: 滤纸片法 筛选 活性物 棉花黄萎病菌 抑制效果, 滤纸片法 筛选 活性物 棉花黄萎病菌 抑制效果

Abstract:

The effect of explants collection time, different sterilization methods, antibiotics to inner bacteria, different medium and growth regulators on adventitious buds regeneration of Ziziphus jujube ‘Junzao’ using nodal explants from mature trees was studied and evaluated to establish a high adventitious buds regeneration system for the use of genetic transformation technology for genetic improvement. The explants were collected in May was best compare to other period. Intermittent sterilization method and adding 480 mg/L penicillin were optimum to control the contamination. Highest regeneration rate was obtained on 1/2MS with TDZ 0.5 mg/L, IBA 0.2 mg/L with the nodal explants inoculation straightly.