欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (31): 152-155.

所属专题： 食用菌； 食用菌； 园艺

• 林学 园艺 园林 • 上一篇    下一篇

基于线粒体序列特征的茶树菇及杨柳田头菇分离物快速鉴定

陈卫民 赵永昌   

  • 收稿日期:2011-07-04 修回日期:2011-08-10 出版日期:2011-12-05 发布日期:2011-12-05
  • 基金资助:

    现代农业产业技术体系建设专项资金资助

he Fast Identification of the Isolation of two Confusing Species A. aegerita and A. salicacola Based on the Mitochondrial Sequences

  • Received:2011-07-04 Revised:2011-08-10 Online:2011-12-05 Published:2011-12-05

PDF (PC)

991

摘要：

此试验拟探寻特异的DNA序列，快速鉴定茶树菇（Agrocybe aegerita）及其形态上易混淆种，给种质资源收集及利用提供有效工具。本研究以云南采集到的18个菌株为材料，其中5株为A. aegerita，13个为A. salicacola，并对线粒体小亚基V9区进行序列分析。结果发现A. aegerita比A. salicacola多1个约50碱基的插入片段，而其他序列组成一致。根据此特点，在插入区筛选到1个特异DraⅠ酶切位点。通过对18个菌株V9区PCR产物进行酶切，发现A. aegeritaV9片段能完全被切开，而A. salicacola V9片段则为完整。根据此酶切方法，可以高效鉴定A. aegerita及A. salicacola 2个近似种，为食用菌遗传及育种研究提供了重要的基础。

关键词: 黄连木, 黄连木, 花粉萌发率, 最佳条件

Abstract:

The aim was to explore special DNA sequences, and identify the confusing species of A. aegerita in morphology for providing an efficient tool for the resource collection and utilization. In this study, 18 strains including 5 A. aegerita and 13 A. salicacola collected in Yunnan Province were investigated on their small mitochondrial V9 sequences. The result showed the identical sequences were found in 2 species except one insertion segment in A. aegerita compared with A. salicacola. Based on the sequence characterization, one unique enzyme digestion site of DraⅠ was found in insertion segment, and the verification on the V9 PCR products of 18 strains confirmed that segments of A. aegerita were digested, and converse result occurred in that of A. salicacola. According to the method of enzyme digestion, 2 species could be efficiently distinguished, and it provided an important basis for genetics and breeding of edible mushroom.