关键词: 氨基酸分析, 氨基酸分析

Abstract: In order to study the pollen preservation methods of Macadamia and provide a theoretical basis for the artificial pollination to increased production and cross breeding of parental combination, the pollen of Macadamia cultivars D was used as material, three drying methods include natural drying, drying in dishes and drying in 40 ℃ oven were used. The pollen were drying in the 40 ℃ oven for 2 h, 6 h, 10 h and 24 h respectively, then preserved in 4 ℃ for 5 d, 10 d, 15 d, 20 d, 25 d and 30 d, the pollen germination rate were determined in agar culture. The result showed that there were no significant differences of pollen germination rates between natural drying and drying dishes, but they all significantly higher than 40 ℃ oven. The pollen dried in natural and preserved 20 days can be used for artificial pollination. The germination rate of macadamia pollen which dried in drying dishes for 2 hours and 6 hours and then preserved 20 days were higher than 15%, these pollen can be used in production. The germination rate of macadamia pollen which dried in 40 ℃ oven were lower than 15% and have no value for artificial pollination. The germination rate of macadamia pollen which dried in natural for 2 hours and preserved in 4 ℃ for 5 days was 42.24%, it was the highest. Considering the convenience of environment regulation, the drying dishes were the best choice in production.