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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (8): 82-86.

所属专题： 生物技术

• 水产 渔业 • 上一篇    下一篇

泥鳅激素浸泡催产试验的初报

胡廷尖 王雨辰 刘士力 周志明 徐海圣 陈丰刚 李倩 宋竞时   

  • 收稿日期:2011-08-31 修回日期:2011-09-30 出版日期:2012-03-15 发布日期:2012-03-15
  • 基金资助:

    湖州市科技局新农村建设计划项目;浙江省公益性计划项目

A Preliminary Report on Exogenous Hormones Soaking Experiment of Loach

  • Received:2011-08-31 Revised:2011-09-30 Online:2012-03-15 Published:2012-03-15

PDF (PC)

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摘要：

为了寻找一种简易的泥鳅催产方法。浸泡催产激素有绒毛膜促性腺激素(HCG)、促黄体素释放激素类似物(LHRH-A2)和地欧酮(DOM)，以HCG250 IU+LHRH-A22 μg+DOM1 mg/尾为基本剂量(X)，食盐作为浸泡助渗剂，食用色拉油为消泡剂。分别进行不同浸泡时间、浓度和盐度的泥鳅催产试验。累计激素浸泡催产种鳅13.987 kg，900尾，获受精卵80.218万粒，其催产率在0%~100%，平均催产率为34.66%；得泥鳅水花苗71.42万尾。孵化率为在45.6%~93.3%之间，孵化率平均值为81.21%。激素浸泡催产率高达80.0%，激素浸泡催产获得泥鳅受精卵的孵化率和注射激素催产获受精卵的孵化率差别不大，说明利用激素浸泡泥鳅催产方便省时，是一种有前途的新方法。浸泡催产激素有绒毛膜促性腺激素（HCG）、促黄体素释放激素类似物（LHRH-A2）和地欧酮（DOM），按照HCG250IU+LHRH-A22μg+DOM1mg/尾基本剂量（X）；食盐为浸泡助渗剂，食用色拉油为消泡剂。2011年6月15日至20日，笔者分别进行不同浸泡时间、浓度和盐度的泥鳅催产试验。累计激素浸泡催产种鳅13.987kg，900尾，获受精卵84.826万粒，其催产率在 0.00%～80.0%，得泥鳅水花苗71.42万尾。孵化率为在45.6%～93.3%之间，孵化率平均值为81.21%。

关键词: BP人工神经网络, BP人工神经网络

Abstract:

The objective of this study is to find a simple method to induce spawning. Exogenous hormones in this experiment include HCG and LHRH-A2 and DOM. Every loach is in accordance with basic amount of HCG250 IU and LHRH-A22 μg and DOM1 mg. Salt is used as aided agent for soaking and salad oil is used to eliminate foam. We studied the effect of different soaking time and different concentrations and different salinity on propagation of loach. The total amount of loach for soaking experiment is 13.987 kg and 900 loaches were injected into exogenous hormones and 802.18 thousand spawning eggs were collected. The rate of inducing spawning was from 0% to 100%. The average spawning rate was 34.66% and 714.2 thousand loach juveniles were got and the hatching rate was from 45.6% to 93.3%. The average value of hatching rate was 81.21%. The rate of induce spawning induced by exogenous hormones is 80% and there is no significant difference on hatching rate between injection hormones and hormones soaking method. This study indicates that hormones soaking method is likely a new convenient, time-saving and promising method for loach.