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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (8): 87-89.

所属专题： 生物技术

• 水产 渔业 • 上一篇    下一篇

Pb2+对水丝蚓的急性毒性及超氧化物歧化酶活性的影响

赵双菁 李艳秋 柳郁滨 范学铭   

  • 收稿日期:2011-11-16 修回日期:2011-12-08 出版日期:2012-03-15 发布日期:2012-03-15

Effect of Pb2+on Limnodilus claparedianu Acute Toxicity and Superoxide Dismutase Activity

  • Received:2011-11-16 Revised:2011-12-08 Online:2012-03-15 Published:2012-03-15

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摘要：

为了研究重金属Pb2+污染的水溶液对水丝蚓造成的胁迫程度。在(22±0.5)℃温度下：（1）采用静水生物测试法测定了不同浓度的Pb2+溶液对水丝蚓的急性毒性，实验结果表明：Pb2+溶液对水丝蚓处理48 h后半致死浓度(LC50)为9.488 μL/L；（2）用检测酶的方法对Pb2+溶液处理48 h后的水丝蚓进行超氧化物歧化酶(SOD)的测定，数据表明：Pb2+对动物的SOD活性影响显著(P＜0.05)。当药物浓度范围在2.000~8.653 μL/L时，SOD活性上升；当药物浓度达到12.481 μL/L后，SOD活性急剧下降。说明Pb2+对水丝蚓可以造成危害，并致其死亡。证明了重金属对水生生物和生物链的影响，对生态环境造成威胁。

关键词: 采后生理, 采后生理

Abstract:

(College of Life Science and Technology, Harbin Normal University, Harbin 150025) Abstract: This article reported on water Pb2+ pollution of heavy metals in aqueous earthworm extent of stress caused. and adopted an acute toxicity method to measure the semi-lethal concentration (LC50) of Limnodilus claparedianus that were disposed in Pb2+ with different concentrations. The variation of L. claparedianus SOD activity toxic was tested by means of enzyme activity. The results showed that the semi-lethal concentration (LC50) of 48 hours was 9.488 μL/L. Pb2+ had a significant effect on L. hoffmeisteri SOD activity (P＜0.05). The SOD activity in the animal specimen increased when they were disposed in low concentration Pb2+ (2.000-8.653 μL/L). But SOD activity in the animal specimen decreased at different levels when concentrations of Pb2+ increased to 12.481 μL/L, a conclusion was achieved that Pb2+ could cause coercion to L. claparedianus and even cause death of animal individuals, and they could also lead to direct and indirect influence on aquatic ecosystems and the subspecies which were fed by L. claparedianus.

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