欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (4): 211-214.

• 林学 园艺 园林 • 上一篇    下一篇

栽培条件对半夏叶片POD、SOD、CAT活性的影响

章艳玲 程纪伦 李关荣   

  • 收稿日期:2011-08-31 修回日期:2011-11-23 出版日期:2012-02-05 发布日期:2012-02-05

Effects of Cultivation Conditions on the Activity of POD, SOD and CAT in the Leaf of Pinellia ternate breit

  • Received:2011-08-31 Revised:2011-11-23 Online:2012-02-05 Published:2012-02-05

PDF (PC)

748

摘要：

为了筛选能提高半夏叶片POD、SOD、CAT活性的栽培条件，用愈创木酚法、NBT光化还原抑制法和紫外分光光度法分别测定了不同基质不同施肥条件下半夏叶片中过氧化物酶（POD）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性。结果表明：半夏叶片POD酶活性受基质影响不明显，但不同施肥处理对其活性影响极显著，其中施用1/2MS+K+P（KH2PO4 100 mg/L）的处理半夏叶片POD酶的活性最强；半夏叶片中SOD和CAT酶的活性受不同基质的影响比较明显，其中以珍珠岩为基质栽培的半夏叶片中两种酶的活性均显著高于以土壤为基质栽培的半夏；不同施肥处理对这两种酶的活性也有影响，其中施用1/2MS+K+P（KH2PO4 100 mg/L）的处理半夏叶片SOD酶的活性最强。栽培半夏以珍珠岩为基质、施用1/2MS+K+P（KH2PO4 100 mg/L）的处理对于提高半夏叶片中POD、SOD、CAT 3种同工酶的活性为佳。

关键词: 非热杀菌技术, 非热杀菌技术

Abstract:

To screen out a Cultivation Condition that can increase the Activity of POD，SOD and CAT in the Leaf of Pinellia ternate breit .Measurements of POD, SOD and CAT activity in the leaf of Pinellia ternate breit cultivated under different treatment were done with gaiacol, NBT and Ultraviolet spectrophotometry. The results show that the difference in leaf POD activity of Pinellia ternate breit cultivated in the two media was insignificant; but different amount of fertilizers had very significant difference on POD activity, with 1/2MS+K+P (KH2PO4 100 mg/L) being the highest; the difference in leaf SOD and CAT activity of Pinellia ternate breit cultivated in the two media was significant, activity of the two enzymes in perlite is very significantly higher than that in soil; different amount of fertilizers had some influence on SOD and CAT activity too, with 1/2MS+K+P (KH2PO4 100 mg/L) being the highest in SOD activity. The best cultivation that can enhance the POD, SOD, CAT activity in the leaf of Pinellia ternate breit is fertilizing with 1/2MS+K+P (KH2PO4 100 mg/L) in perlite.