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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (5): 48-52.

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

不同剂量党参、淫羊藿提取物对青脚麻鸡脾脏组织结构影响

周金星 李升和 宁康健 金光明   

  • 收稿日期:2011-09-06 修回日期:2011-10-08 出版日期:2012-02-15 发布日期:2012-02-15
  • 基金资助:

    安徽科技学院重点学科基金项目

Effect on the Tissue Structure of Partridge Shank Chicken’s Spleen with Different Dosage Extraction

  • Received:2011-09-06 Revised:2011-10-08 Online:2012-02-15 Published:2012-02-15

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摘要：

为研究不同剂量党参、淫羊藿对青脚麻鸡脾脏组织结构的影响。将420只14日龄青脚麻鸡随机均分7组，分别为饮用党参和淫羊藿提取物大(2 g/L)、中(1 g/L）、小(0.5 g/L）浓度组和对照组(饮用自来水）。于28，35，42，49，56日龄时早晨空腹随机抽样12只解剖，迅速取脾脏标本，称重后Bouin液常温固定24 h，乙醇脱水、二甲苯透明、石蜡包埋，用LEICA-RM-2128型切片机切成5 μm切片，HE染色，中性树胶封片，显微观察，OLYMPNS-CH30型显微摄影系统显微摄影。结果表明，党参和淫羊藿各试验组鸡脾脏红髓和白髓界限清晰，脾小体明显，动脉周围淋巴鞘数量增多，动脉周围淋巴鞘增厚，其中以2 g/L组结构最为明显。说明在饮水中添加党参、淫羊藿提取物均对青脚麻鸡脾脏的发育有促进作用。

关键词: 释磷性能, 释磷性能

Abstract:

To study the effect on the tissue structure of Partridge Shank Chicken’s spleen with different dosage extraction from Pilose Asiabell Root and Herba Epimedii Brevicornus. 420 Partridge Shank Chickens of 14-day-age were randomly divided into seven groups. Control groups and experiment group were drunk water and different doses of extract from Pilose Asiabell Root and Herba Epimedii Brevicornus, Setting the Ⅰ group as contrast; setting the Ⅱ, Ⅲ, Ⅳ and Ⅴ,Ⅵ,Ⅶ group as small(0.5 g/L), middle(1.0 g/L), large(2.0 g/L) doses of extraction from Pilose Asiabell Root and Herba Epimedii Brevicornus. In 21th, 28th, 35th, 42th, 49th and 56th day morning, select 12 chicken for dissection before they were fed, taking anatomical specimens quickly and keeping it under Bouin liquid in normal atmospheric temperature 24 hours to be fixed, then, Dehydrated by ethanol, Make it transparent by xylene, paraffin embedding, Cutting it into 5um tire-road friction coefficient with the LEICA-RM-2128 type slicing machine, HE dying, sealing in piece with neutral gum, OLYMPNS-CH30 microstructure for observation, making the microstructure photography with microscopic photography system. The result show that experimental group of Pilose Asiabell Root and Herba Epimedii Brevicornus chicken spleen red pulp and white pulp had a clear boundaries, spleen bodies is obvious, the number of sheath which around arterial has increased, periarterial lymphatic sheath thickening. The 2 g/L group is obvious. The results account for that Pilose Asiabell Root and Herba Epimedii Brevicornus can promote the development of the spleen.