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Abstract:

ST cell is a continuous cell line which widely used in veterinary vaccine production. This study used a new type of tidal bioreactor, Bellocell, for the ST cell culture. ST cells were amplified by 3L roller bottle. When the cell number reached 1.07×108, dispersed cells were seeded into tidal reactor Bellocell? -500, with 500ml culture medium, and cultivated for 7days. In each day, the cell number, pH and glucose values change were monitored. The glucose consumption increased by days, and after 48 hours new medium should be change per day in order to maintain normal cell growth. During the observation period from 1d to 6d, ST cells multipled in the 1.2-2.4 times per day and the cell number reached the peak of 3.17×109 at the 6th day, which was 30 times of the initial seeds. When the cells number reached 109, the ST cells on the carrier showed spindle-shaped, uniform size in microscope, result of trypan blue staining confirmed that cells were surviving. This study successfully achieved a high density culture for the ST cell，that would provide a simple and efficient way for the production of veterinary vaccine with the ST cell.