欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (5): 23-27.

所属专题： 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

血清11型鸭疫里默氏菌的分离鉴定

杨晓辉 程龙飞 陈红梅 施少华 傅光华 万春和 黄瑜   

  • 收稿日期:2011-10-09 修回日期:2011-11-15 出版日期:2012-02-15 发布日期:2012-02-15
  • 基金资助:

    现代农业产业技术体系建设专项资金

Isolation and identification of serotype 11 Riemerella anatipestifer

  • Received:2011-10-09 Revised:2011-11-15 Online:2012-02-15 Published:2012-02-15

PDF (PC)

870

摘要：

从同一发病鸭场间隔4天分离到两株鸭疫里默氏菌（RA1156和RA1157），为比较他们的差异，采用平板凝集试验和琼扩试验鉴定血清型，纸片法测定耐药性，动物试验测定致病性。结果显示，两株鸭疫里默氏菌的血清型相同，均为11型；对樱桃谷鸭的致病性相同；对大多数药物的敏感性相同，RA1156对庆大霉素和青霉素敏感，而RA1157对庆大霉素和青霉素耐药。本试验确认了11型鸭疫里默氏菌在福建的存在，增添了福建省鸭疫里默氏菌的流行病学资料；与实验室人工诱导相比，体内的鸭疫里默氏菌更容易产生耐药性。

关键词: 宁乡县, 宁乡县

Abstract:

Two strains of Riemerella anatipestifer (RA1156 and RA1157) had been isolated from the same duck farm interval of four days. To compare the differences of the two strains, plate agglutination and agar gel precipitation tests were used to identify the serotypes of them, Kirby-Bauer method was used to detect the drug sensitivities, and animal testing was used to determine the pathogenic of them. The results showed that both of two strains were serotype 11, had the same pathogenic to duck, and had the same sensitivities to most drugs. Strain RA1156 was sensitive to Gentamicin and Penicillin, while strain RA1157 was resistant to these two drugs. This test confirmed the presence of serotype 11 Riemerella anatipestifer in Fujian province and added the epidemiological data of Riemerella anatipestifer. Compared with laboratory artificially induced, the strain in vivo might get the resistance to drug easier.