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中国农学通报 ›› 2012, Vol. 28 ›› Issue (17): 143-146.doi: 10.11924/j.issn.1000-6850.2011-3618

所属专题: 生物技术

• 水产 渔业 • 上一篇    下一篇

Hg2+对水丝蚓的急性毒性及超氧化物歧化酶活性的影响

孙娜 王宇佳 柳郁滨 范学铭   

  • 收稿日期:2011-12-03 修回日期:2012-01-06 出版日期:2012-06-15 发布日期:2012-06-15

Effects of Hg2+on Limnodilus claparedianu Acute Toxicity and Superoxide Dismutase Activity

  • Received:2011-12-03 Revised:2012-01-06 Online:2012-06-15 Published:2012-06-15

摘要:

为探究水生环境对Hg2+的承载限度。在实验室条件下,不同浓度Hg2+对水丝蚓胁迫48 h后的急性毒性及超氧化物歧化酶(SOD)活性的变化。结果表明:在静水实验条件下,Hg2+溶液对水丝蚓处理48 h后半致死浓度(LC50)为9.659 μL/L;Hg2+对动物SOD活性影响显著(P<0.05)。当Hg2+浓度在8.000~9.241 μL/L范围时,SOD活性显著上升,当浓度增加至16.449 μL/L时,SOD的活性呈急剧下降趋势。表明在不同浓度的Hg2+迫下,水丝蚓出现不同程度的中毒反应,甚至死亡。

关键词: 弛豫时间, 弛豫时间

Abstract:

In order to clarify the aquatic environment to the bearing limit Hg2+. The author of this article adopted an acute toxicity method to measure the semi-lethal concentration (LC50) of Limnodilus claparedianus that were disposed in Hg2+ with different concentrations. The variation of L. claparedianus SOD activity toxic was tested by means of enzyme activity in this paper. The results showed that the semi-lethal concentration (LC50) of 48 hours was 9.659 μL/L. Hg2+had a significant effect on L. hoffmeisteri SOD activity (P<0.05). The SOD activity in the animal specimen increased when they were disposed in low concentration Hg2+ (8.000~9.241 μL/L). But SOD activity in the animal specimen decreased at different levels when concentrations of Hg2+ increased to 10.674 μL/L, a conclusion was achieved that Hg2+ could cause coercion to L. claparedianus and even cause death of animal individuals, and they could also lead to direct and indirect influence on aquatic ecosystems and the subspecies which were fed by L. claparedianus.

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