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中国农学通报

中国农学通报 ›› 2005, Vol. 21 ›› Issue (6): 102-102.

所属专题： 生物技术

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勿忘我的组织培养研究

王家福,齐永鑫   

  • 出版日期:2005-06-05 发布日期:2005-06-05

A Study of Limonium sinuatum Tissue Culture

Wang Jiafu, Qi Yongxin   

  • Online:2005-06-05 Published:2005-06-05

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摘要： 以勿忘我幼嫩花序为外植体，进行组织培养快繁技术的研究。试验结果表明：在起始培养基附加20 mg/L的苯甲酸钠对抑制污染有一定作用。其最适的芽增殖培养基为：MS+BA1.0+NAA0.1+3%砂糖，交替附加0.5 mg/LGA3的效果为好，诱导生根培养基为1/2MS+NAA0.4+2%砂糖为佳。

Abstract: Using the young inflorescence as material, tissue culture of L.sinuatum was studied. The results showed that the contamination can be effectively controlled on the initiated medium containing 20mg/L sodium benzoate. The most suitable medium for bud multiplication was MS+BA1.0 mg/L+NAA0.1 mg/L+ sucrose 3%, adding 0.5 mg/LGA3 alternately .The optimal medium for rooting was 1/2MS+NAA0.4 mg/L+ sucrose 2%.