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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (29): 39-43.

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

不同来源牛体外胚胎与牛子宫内膜细胞共培养体系的建立

张彧 陈利平 梁鸿斌 李娅杰 赵雅琦 阎芃 杨菲 倪和民 郭勇   

  • 收稿日期:2012-03-16 修回日期:2012-03-25 出版日期:2012-10-15 发布日期:2012-10-15
  • 基金资助:

    自然科学基金“IFNτ协调Galectin 15对绵羊体外胚胎着床调节机制的研究”

Establishing an Effective Way to Co-culture the in-vitro Bovine Blastocysts from Different Methods With Their Uterine Endometrium Cells

  • Received:2012-03-16 Revised:2012-03-25 Online:2012-10-15 Published:2012-10-15

PDF (PC)

613

摘要：

为了初步建立有效的牛胚胎与子宫内膜细胞的体外共培养体系。本实验分别将体外受精第7天后的胚胎和孤雌激活，与培养7天的胚胎与子宫内膜细胞共培养，在48 h和72 h后分别统计体外受精实验组和孤雌激活实验组的孵化率。实验结果表明：在本实验室培养条件下，在与子宫内膜细胞共培养48 h后，体外受精胚胎和孤雌发育胚胎在孵化率上存在显著差异(40±5)%、(25±5)%，(P＜0.05)；与子宫内膜共培养72 h后，孵化率无显著差异(70±8.66)%，(65±5)%，(P＞0.05)。本实验证明了在体外环境下，牛体外胚胎与子宫内膜细胞共培养后能够正常孵化，可以初步建立起牛体外胚胎与子宫内膜细胞的体外共培养体系，并为进一步研究该体系对牛体外胚胎培养质量的影响奠定一些基础。

关键词: 畜牧、动物医学, 畜牧、动物医学

Abstract:

In this study, it was initiated to establish an effective way to co-culture the bovine blastocysts and their uterine endometrium cells in vitro. Here, total 7 days in vitro fertilized (IVF) or 7 days parthenogenetic (PA) bovine blastocysts were put into the quadripuntal broad with p5 uterine endometrium cells. The results showed that, after being co-cultured with the uterine endometrium cells for 48 h, the hatching rate of IVF blastocysts was significantly higher than that of PA group (40±5)% vs (25±5)%, P＜0.05), but after being co-cultured for 72 h, there was no significant differences of hatching rate between them (70±8.66)% vs (65±5)%, P>0.05). In conclusion, these results suggested that such kind of in vitro co-culture system between those in vitro produced bovine blastocysts and the uterine endometrium cells, be a potential and effective way for further investigating the quality of bovine embryos.