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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (33): 307-310.

• 食品 营养 检测 安全 • 上一篇    下一篇

Tween 80与Span 80在食品添加剂微生物学检验中的应用

田金秋 张萍 郑大威 王晓萌 钟儒刚   

  • 收稿日期:2012-03-27 修回日期:2012-06-04 出版日期:2012-11-25 发布日期:2012-11-25
  • 基金资助:

    国家自然科学基金项目（21107005）资助;教育部博士点基金项目（20111103120011）资助;北京工业大学博士科研启动基金资助

Application of Tween 80 with Span 80 in Dilution Systems Used for Oily Food Additives Microbial Examination

  • Received:2012-03-27 Revised:2012-06-04 Online:2012-11-25 Published:2012-11-25

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摘要：

根据国标GB 4789规定方法对富含油脂类食品进行微生物学检验时，由于样品梯度稀释困难，往往会造成取样量不准确，对检验结果造成不良影响。为此将表面活性剂按照一定的复配比例应用于富含油脂类食品的梯度稀释过程中，并评价样品乳化效果及其对微生物生长的影响。研究结果表明，v(Tween 80):v(Span 80)=1:1，添加浓度为1%~5%时，对微生物生长没有促进与抑制作用，在对富含油脂类产品（含油量在95%以上）进行梯度稀释时，添加浓度为2%即可达到较好的乳化效果。该方法建立了更为完善的样品前处理方式，能够方便、准确地取样，对于改善富含油脂类食品的微生物学检验具有一定的参考价值。

关键词: 净初级生产力, 净初级生产力

Abstract:

The oily food additives are difficult to be exactly diluted using sterilized physiological saline or phosphate buffer solution according to GB 4789 methods to detect the microbial contaminations. And the inappropriately diluted manner affects the result accuracy directly. This research had established a proper dilution method through application of compound surfactants in the pretreatment processions. The emulsifying powers and microbial growth effects of Tween-80 compounded with Span-80 had been studied. The results showed that added compound surfactants of Tween-80 with Span-80 (1:1) in the concentrations from 1% to 5% had no effects on microbial growth. And the oily food additives (oil content >95%) had been relatively stable emulsificated by only 2% of compound surfactants in the dilution system. This research provided convenient and accurate sample pretreatment techniques which were especially useful for oily foods to detect the microbial contaminations.