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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (33): 296-301.

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响应面法优化超声辅助提取桑叶总黄酮工艺研究

贺伟强   

  • 收稿日期:2012-04-05 修回日期:2012-05-13 出版日期:2012-11-25 发布日期:2012-11-25
  • 基金资助:

    嘉兴蚕桑资源降血糖功效的研究与开发

Optimization of Technology for Ultrasonic-assisted Extraction of Total Flavonoids from Mulberry leaves by Response Surface Methodology

  • Received:2012-04-05 Revised:2012-05-13 Online:2012-11-25 Published:2012-11-25

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摘要：

为研究不同提取条件对桑叶总黄酮提取率的影响，以便优化桑叶总黄酮的提取工艺条件。选取液料比、超声时间和超声温度为影响因子，在单因素试验的基础上，进行3因素3水平的Box-Behnken中心组合试验设计。以桑叶总黄酮提取率为响应值，进行响应面分析，优化超声辅助提取桑叶总黄酮的提取条件。试验结果表明，超声辅助提取桑叶总黄酮的最佳工艺条件为：75%乙醇，在液料比为50:1、超声温度64℃，超声45 min后，桑叶总黄酮提取率理论值可达到4.29%，验证值为4.23%。实验结果为确定桑叶总黄酮的超声辅助提取工艺提供了实验依据。

关键词: 槐定碱, 槐定碱

Abstract:

In order to study the different parameter of total flavonoids from Mulberry leaves, the best extracting process was ensured. The ratio of liquid to solid, the ultrasonic time and ultrasonic temperature were selected on the basis of single factor experiment, 3 factors 3 levels of Box-Behnken center combination experimental design, the extraction of rate of Mulberry leaves was used as response value for response surface analysis, and the flavonoids Ultrasonic-assisted extraction conditions were optimized. The results showed that the optimal preparation procedure of total flavonoids consisted of 75% ethanol, the ration of liquid to solid 50:1, extraction temperature 64℃, extraction time 45 min. Under the best extraction conditions, the theoretical extraction rate of the total flavonoids can reach 4.29%, validation value is 4.23%. The experiment result could provide basic and instruction data for the Ultrasonic-assisted extraction technology of total flavonoids from Mulberry leaves.