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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (29): 20-24.

所属专题： 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

着床前小鼠孤雌囊胚及正常囊胚上Wnt-3a的差异表达研究

林皓 王灏 刘石磊 刘振伟 刘云海 倪和民 郭勇   

  • 收稿日期:2012-04-09 修回日期:2012-05-03 出版日期:2012-10-15 发布日期:2012-10-15
  • 基金资助:

    国家自然基金面上资助

Expression of Wnt-3a on the Pre-implantation of Both Mouse Parthenogenetic and Normal Embryos

  • Received:2012-04-09 Revised:2012-05-03 Online:2012-10-15 Published:2012-10-15

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摘要：

为了研究Wnt-3a在小鼠着床前，孤雌囊胚和正常胚胎上的表达差异情况。采用激光扫描共聚焦显微技术和半定量RT-PCR检测方法，对孤雌囊胚和正常囊胚上Wnt-3a的表达，以及分布情况进行定性和定量的检测。利用激光扫描共聚焦显微技术检测发现：在着床前的小鼠孤雌囊胚和正常囊胚上都有Wnt-3a蛋白质的表达，且只在滋养层细胞中表达。通过半定量RT-PCR检测发现，着床前小鼠孤雌胚胎与正常胚胎相比，Wnt-3a的转录水平没有显著性差异。所以Wnt-3a在着床前小鼠孤雌胚胎和正常胚胎中都有表达，而且其转录水平在这2种胚胎中没有显著性差异。

关键词: 立地条件, 立地条件

Abstract:

This experiment was aimed to investigate the expression of Wnt-3a on the pre-implantation of both mouse parthenogenetic and normal embryos. In this study, Wnt-3a protein and mRNA be detected and analyzed by laser scanning confocal microscope and semi-quantitative RT-PCR. The results of laser scanning confocal microscope were shown that Wnt-3a protein was only detected on trophoblast cell of both normal and parthenogenetic embryos. The results of semi-quantitative RT-PCR were shown that the transcription level of Wnt-3a have no distinct difference between normal and parthenogenetic embryos. Collectively, it could be concluded that there was expression of Wnt-3a on both normal and parthenogenetic embryos, and there exists no distinct difference of transcription level between normal and parthenogenetic embryos.