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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (29): 125-130.

所属专题： 生物技术； 水产渔业

• 水产 渔业 • 上一篇    下一篇

草鱼MyoG基因的克隆及其组织表达谱分析

宫佳琦 李瑞文 景睿松 吕彦红 郑玉才 周继术 林亚秋   

  • 收稿日期:2012-04-09 修回日期:2012-06-08 出版日期:2012-10-15 发布日期:2012-10-15

Cloning and Expression Profiling of MyoG Gene of Myogenin

  • Received:2012-04-09 Revised:2012-06-08 Online:2012-10-15 Published:2012-10-15

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摘要：

为了获得草鱼(Myogenin) MyoG基因序列，阐明其在草鱼不同组织的表达规律。根据鲤鱼(Cyprinus carpio)基因MyoG序列(AB012881)设计引物，通过RT-PCR技术克隆草鱼MyoG的cDNA全序列，并对cDNA序列的氨基酸同源性、理化性质等进行了预测和分析。同时利用半定量RT-PCR分析草鱼MyoG基因在草鱼不同组织中的mRNA表达特性。结果表明：得到草鱼MyoG序列789 bp，开放阅读框(ORF)序列762 bp， GenBank登陆号为JQ793897。编码253个氨基酸，具有MyoD家族基因的典型性碱性螺旋-环-螺旋(Basic helix-loop-helix，bHLH)结构，与斑马鱼、鲤鱼、虹鳟、大西洋鲑等同源性较高，为73%~95%，但与哺乳动物和禽类如人、小鼠、猪、牛和鸡的同源性较低，为53%~57%。在草鱼红肌、白肌、脂肪、肝胰脏、肾脏、脑、肠、心脏、鳃中均检测到MyoG基因的表达，并且在白肌中表达量显著高于其他组织(P＜0.05)，在脑、肝胰脏和鳃中亦有较高水平表达。成功获得草鱼MyoG基因序列，并在白肌中的表达显著高于其他组织，为研究MyoG在草鱼肌肉发育过程中的作用提供基础资料。

关键词: 细胞型生态人居, 细胞型生态人居

Abstract:

In order to obtain the (Myogenin) MyoG gene sequence of grass carp and its expression pattern in the different muscle tissue. PCR primers were designed according to Cyprinus carpio MyoG gene sequence (AB012881). Using RT-PCR method, we amplified MyoG gene sequence. The analysis and acquired sequences. We also analyzed the mRNA expression character of MyoG gene, using semi-quantitative RT-PCR, in grass carp different tissue. The results showed that grass carp MyoG gene cDNA was 789 bp (GenBank accession No. JQ793897), with an ORF of 762 bp. The cDNA encoded 253 amino acids peptides, with typical MyoD gene family structure trait of Basic helix-loop-helix (b-HLH). Compared with Zebrafish, common carp, rainbow trout and atlantic salmon, the homology of amino acid sequences were higher (73%-95%), but lower in some mammals and poultry (53%-57%). The MyoG gene expression was observed in red muscle, white muscle, fat, hepatopancreas, kidney, brain, intestines, heart and gill and the expression level of MyoG gene in white muscle was significantly higher than that of other tissues studied (P＜0.05), and in brain, hepatopancreas and gill also have a high level expression. We successfully obtained the MyoG gene sequence of grass carp, and its expression level in white muscle was significantly higher than that of other tissues. We offered a initial value for the role of MyoG on the muscle tissue development of grass carp.