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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (32): 63-67.

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

猪附红细胞体ORF4蛋白的空间结构与B细胞抗原表位预测

单思 巴彩凤   

  • 收稿日期:2012-04-13 修回日期:2012-05-24 出版日期:2012-11-15 发布日期:2012-11-15
  • 基金资助:

    辽宁省科技基金资助项目

Prediction of the Spatial Structure and B-cell Epitopes of Mycoplasma suis ORF4 Protein

  • Received:2012-04-13 Revised:2012-05-24 Online:2012-11-15 Published:2012-11-15

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摘要：

为了预测猪附红细胞体(Mycoplasma suis)ORF4蛋白的空间结构和B细胞抗原表位。选择Genebank序列号AJ504999中ORF4基因，应用生物信息软件和互联网在线程序分析ORF4的氨基酸序列预测二级结构、三级结构、亲水性、柔韧性、抗原指数及表面可及性。结果表明：ORF4蛋白二级结构没有α-螺旋，其中柔性结构以β-转角为主，潜在的优势B细胞抗原表位区段主要分布在28~34，54~61氨基酸区段。本研究预测了ORF4蛋白空间结构和B细胞抗原表位，为猪附红细胞体表位疫苗的设计研发提供了指导意义。

关键词: 长沙市, 长沙市

Abstract:

To predict the spatial structure and B-cell epitopes of Mycoplasma suis ORF4 protein. The secondary structure, tertiary structure, hydrophilicity, flexible region, antigenic index and surface probability were predicted through the nucleotide sequence of AJ504999-ORF4 were analyzed by bioinformatics software and internet routine. The results showed that the potential B cell antigenic epitopes of Mycoplasma suis ORF4 protein were located at 28-34, 54-61aa, whose the secondary structure were without α-helix but rich in β-pleated sheet. Prediction of the spatial structure and B-cell epitopes of Mycoplasma suis ORF4 protein might laid the basis for studies of the design of epitopes vaccine.