欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (36): 107-112.

所属专题： 生物技术

• 农学 农业基础科学 • 上一篇    下一篇

响应曲面法优化罗布麻愈伤组织中绿原酸的提取工艺

邓倩 王芳 刘小锐 计巧灵   

  • 收稿日期:2012-05-02 修回日期:2012-06-21 出版日期:2012-12-25 发布日期:2012-12-25
  • 基金资助:

    亚麻与罗布麻体细胞杂交及其种质的耐盐性遗传研究

RSM in Extraction Process Optimization for Yield of Chlorogenic Acid in Callus of Apocynum venetum

  • Received:2012-05-02 Revised:2012-06-21 Online:2012-12-25 Published:2012-12-25

PDF (PC)

706

摘要：

通过优化罗布麻愈伤组织中绿原酸的提取工艺，为进一步探索规模化提取罗布麻愈伤组织中的绿原酸奠定前期工作基础。以罗布麻愈伤组织中绿原酸得率为指标，通过HPLC法测定并比较，得出最佳提取方法；在单因素试验的基础上，结合响应曲面法优化提取绿原酸的工艺。从罗布麻愈伤组织中提取绿原酸的最佳方法是超声波辅助提取法；在单因素试验的基础上，结合响应曲面法优化超声波辅助提取绿原酸的最佳工艺条件为：提取时间40 min，提取功率40 W，料液比1:40，绿原酸最高得率为0.4911 mg/g(D.W)，提取时间和提取功率的交互作用对得率有显著影响。建立的二次多项式数学模型对从罗布麻愈伤组织中提取绿原酸具有重要的指导作用。

关键词: RAPD, RAPD

Abstract:

RSM in extraction process optimization for yield of chlorogenic acid (CGA) in callus of Apocynum venetum, the results laid the foundation for further exploration of extracting CGA in the callus on large scale. The yield of CGA was taken as the index, CGA yields in the callus were determined with HPLC and compared, the optimum extraction process was determined. On the basis of single factor analysis test, combined with central composite experimental design principle, response surface methodology (RSM) was used to optimize extraction process. The optimum extraction method from callus of A. venetum was microwave-assisted extraction. On the basis of single factor analysis test, experiments were carried out and combined with RSM, the optimum microwave-assisted extraction process was as follows: extraction time 40min, extraction power 40w, ratio of solid to liquid 1:40, the CGA maximum yield reached 0.4911 mg/g(D.W), interaction between extraction time and extraction power had a significant impact. Establishment of the two polynomial mathematical models had the important role for CGA extraction from callus of A. venetum.