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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (21): 86-91.doi: 10.11924/j.issn.1000-6850.2012-1118

• 农学 农业基础科学 • 上一篇    下一篇

8份剑麻种质亲缘关系的ISSR和RAPD分析

陈涛 杨祥燕 蔡元保 王春田   

  • 收稿日期:2012-03-27 修回日期:2012-05-10 出版日期:2012-07-25 发布日期:2012-07-25
  • 基金资助:

    现代农业产业技术体系建设专项资金

ISSR and RAPD Analysis of Genetic Relationship in 8 Sisal Germplasm Resources

  • Received:2012-03-27 Revised:2012-05-10 Online:2012-07-25 Published:2012-07-25

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摘要：

为了揭示剑麻栽培品种的遗传多样性，利用ISSR和RAPD分子标记技术对8份剑麻种质的亲缘关系进行分析。结果表明，筛选后选用的8条ISSR引物和8条RAPD引物，分别产生了53条和66条扩增条带，其中多态性条带分别为44条和61条，多态性条带百分率分别为83.02%和92.42%。根据2种标记的扩增结果，用UPGMA法对8份剑麻种质进行聚类分析，供试材料之间具有较高的遗传多样性，其品种间遗传相似系数分别为0.59~0.80和0.52~0.76。2个标记的聚类结果基本一致，但有点差异，可将供试的8份剑麻种质划分为2类群，而且2个标记聚类结果呈显著相关性，相关系数为0.70。可见，剑麻种质资源的遗传多样性丰富。

关键词: 过氧化物酶, 过氧化物酶

Abstract:

The objective of this paper is to reveal the genetic diversity of sisal cultivars. The genetic relationships of 8 sisal germplasm resources were analyzed by ISSR and RAPD. Polymorphism was identified by 8 ISSR primers and 8 RAPD primers in the germplasm. 53 bands were generated by ISSR with polymorphic bands accounting for 83.02% and similarity coefficient within 0.59 to 0.80. And 66 bands were generated by RAPD with polymorphic bands accounting for 92.42% and similarity coefficient from 0.52 to 0.76. Using cluster analysis (UPCMA) based on those polymorphic bands amplified with ISSR and RAPD primers, the relationships of 8 sisal species were constructed. The clustering results of RAPD and ISSR markers were similar, but had a little difference. The germplasm were divided into 2 clusters, and the significant correlation between ISSR and RAPD markers was observed (r=0.70). The results revealed that there was rich genetic diversity among resources of sisal.