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中国农学通报 ›› 2015, Vol. 31 ›› Issue (2): 112-117.doi: 10.11924/j.issn.1000-6850.2014-1908

所属专题: 生物技术 水产渔业

• 水产 渔业 • 上一篇    下一篇

不同壳性状虾夷扇贝体腔液三种酶活力的比较

段立柱1,2,黄显雅1,2,胡婉彬1,2,刘敏博1,2,湛垚垚1,2,丁君1,2,郝振林1,2,常亚青1,2,于瑶2,明月2   

  1. (1农业部北方海水增养殖重点实验室,辽宁大连 116023;2大连海洋大学水产与生命学院,辽宁大连 116023)
  • 收稿日期:2014-07-10 修回日期:2015-01-15 接受日期:2014-09-29 出版日期:2015-03-19 发布日期:2015-03-19
  • 通讯作者: 湛垚垚
  • 基金资助:
    国家“863”计划项目“主要养殖双壳贝类良种培育”(2012AA10AA410);国家“948”项目“水产主导品种分子标记辅助育种技术引进与水产育种技术平台的建立”(2011-G12);国家海洋公益行业科研专项“扇贝对虾加工关键技术与设备研发及扇贝养殖生态环境保障技术应用与示范”(2012 05031)。

Comparison of Three Coelomic Fluid Enzymes Activities in Scallop Patinopecten yesoensis

Li Zhuduan1,2,Xian Yahuang1,2,Wan Binhu1,2,Min Boliu1,2,Yao Yaozhan1,2,Jun Ding1,2,Zhen Linhao1,2, Ya Qingchang1,2,Yao Yu2,Yue Ming2   

  1. (1Key Laboratory of Mariculture & Stock Enhancement in North China Sea, Ministry of Agriculture, Dalian Liaoning 116023; 2College of Fisheries and Life Science, Dalian Ocean University, Dalian Liaoning 116023)
  • Received:2014-07-10 Revised:2015-01-15 Accepted:2014-09-29 Online:2015-03-19 Published:2015-03-19

摘要: 研究利用生物化学方法对不同壳性状虾夷扇贝体腔液中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活力进行比较分析。结果表明,不同贝壳形态的虾夷扇贝,其体腔液中SOD、CAT和POD三种酶活力不存在著性差异(P>0.05);不同壳色虾夷扇贝体腔液中,SOD和CAT酶活力存在显著差异(P<0.05),而POD酶活力差异并不显著。研究结果提示,虾夷扇贝的贝壳形态与其体腔液中抗氧化酶活力高低不存在相关性,而虾夷扇贝贝壳的壳色性状与其体腔液中抗氧化酶SOD和CAT活力高低存在一定的相关性。研究结果可为深入研究扇贝贝壳性状积累一定的理论数据,同时也对扇贝的良种选育具有一定的指导意义。

关键词: 聚乙烯醇, 聚乙烯醇, 筛选, 鉴定, 枯草芽孢杆菌, 固定化

Abstract: In order to find the relationship between immune-related enzyme activities and shell traits in scallop Patinopecten yessoensis, superoxide dismutase(SOD), catalase(CAT), Peroxidase(POD) activities of coelomic fluid were examined and compared. From our study, there is no significant difference in SOD, CAT and POD activities among different shell sizes. However, for P. yessoensis in different shell colors, the difference in SOD and CAT activities of coelomic fluid between brown and white is significant (P<0.05), while there is no significant difference in POD activities. Our results suggested that shell color is to some degree correlated with SOD and CAT activities. This study has enriched our knowledge of scallop shell traits and can provide a new direction of scallop selective breeding.